Vinculin Monoclonal Antibody
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Price:
Size:
100 μL
25 μL
- 宿主： Mouse
- 反应性： Human;Mouse;Rat
- 应用性： WB;IHC;IF
| Verified Samples |
Verified Samples in WB: HeLa, 293, Hep G2, NIH/3T3, Mouse heart, Rat heart Verified Samples in IHC: Human breast cancer, Human colon cancer Verified Samples in IF: HeLa, NIH/3T3, C6 |
| Dilution | WB 1:2000-1:4000, IHC 1:200-1:400, IF 1:200-1:400 |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Immunogen | Recombinant human Vinculin protein expressed by E.coli |
| Abbre | Vinculin |
| Synonyms | Vinculin, Metavinculin (MV), VCL |
| Swissprot | |
| Calculated MW | 124 kDa |
| Observed MW | 126 kDa |
| Cellular Localization | Cell membrane, Cell junction, Cytoplasm, Cytoskeleton, Cell projection, |
| Tissue Specificity | Metavinculin is muscle-specific. |
| Concentration | 1 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Protein A/G Purification |
| Research Areas | Cardiovascular, Signal Transduction, Isotype/Loading Controls, Epigenetics and Nuclear Signaling |
| Clone No. | 4D4 |
| Conjugation | Unconjugated |
| Background | Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa,1,066-amino-acid protein which is ubiquitously expressed. Its splice variant,metavinculin (124 kDa),is muscle-specific. |
Western blot with Anti Vinculin Monoclonal Antibody at dilution of 1:3000. Lane 1: HeLa cell lysate, Lane 2: 293 cell lysate, Lane 3: Hep G2 cell lysate, Lane 4: NIH/3T3 cell lysate, Lane 5: Mouse heart tissue lysate, Lane 6: Rat heart tissue lysate.
Immunohistochemistry of paraffin-embedded Human breast cancer using Vinculin Monoclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Human colon cancer using Vinculin Monoclonal Antibody at dilution of 1:1000.
Immunofluorescence analysis of HeLa cells using Vinculin Monoclonal Antibody at dilution of 1:200.
Immunofluorescence analysis of NIH/3T3 cells using Vinculin Monoclonal Antibody at dilution of 1:200.
Immunofluorescence analysis of C6 cells using Vinculin Monoclonal Antibody at dilution of 1:200.
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