Alanine Aminotransferase (ALT/GPT) Activity Assay Kit
中文名称:谷丙转氨酶(ALT/GPT)比色法测试盒

Price:
Size:
96 T (42 samples)
48 T (18 samples)
- 样本类型: 血清(浆);动物组织;细胞;细胞上清
- 检测仪器及波长: 酶标仪(500-520 nm)
- 研究领域: 肝肾功能
为了简化计算过程,可下载该产品计算文件。如使用中有任何问题,请随时联系技术支持
该指标在测定组织或细胞样本时,需要测定样本的总蛋白含量,推荐使用BCA法测蛋白,货号E-BC-K318-M
说明书或网站上未涉及的样本类型请先跟技术支持沟通,可进行售前验证
根据需测样本数,试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量,请点击按钮 计算
检测原理 |
在37°C、pH7.4条件下,谷丙转氨酶(ALT)催化丙氨酸与α-酮戊二酸之间的氨基转换反应,生成丙酮酸和谷氨酸,反应时间过后,加入苯肼,与丙酮酸生成苯腙,苯腙在碱性条件下呈红棕色。
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指标简称 | ALT |
灵敏度 | 0.75 IU/L |
检测范围 | 0.75-72.30 IU/L |
精密度 | 批间差:0.09 | 批内差:5.3% |
样本量 | 10 μL |
检测时间 | 1 h 20 min |
检测仪器及波长 | 酶标仪(500-520 nm) |
保存条件 | 该产品可在 2-8°C避光条件下保存6个月。 |
有效期 | 6个月 |
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E-BC-K236-M | Aspartate Aminotransferase (AST/GOT) Activity Assay Kit | 96 T (43 samples) , 48 T (19 samples) |