Alanine Aminotransferase (ALT/GPT) Activity Fluorometric Assay Kit
中文名称:谷丙转氨酶(ALT/GPT)活性荧光法测试盒

Price:
Size:
96 T (80 samples)
48 T (32 samples)
- 样本类型: 血清(浆);动物组织
- 检测仪器及波长: 荧光酶标仪(激发光波长535 nm,发射光波长587 nm)
- 研究领域: 肝肾功能
该指标在测定组织或细胞样本时,需要测定样本的总蛋白含量,推荐使用BCA法测蛋白,货号E-BC-K318-M
说明书或网站上未涉及的样本类型请先跟技术支持沟通,可进行售前验证
根据需测样本数,试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量,请点击按钮 计算
检测原理 |
谷丙转氨酶(ALT)能够催化丙氨酸和α-酮戊二酸发生转氨基反应,生成丙酮酸和谷氨酸。丙酮酸在丙酮酸氧化酶的作用下生成H2O2,在过氧化物酶作用下,将无荧光探针氧化为有荧光的物质,通过测定体系荧光值的增加量,即可计算出ALT的活性。
|
指标简称 | ALT |
灵敏度 | 0.01 U/L |
检测范围 | 0.01-0.83 U/L |
精密度 | 批间差:9.8% | 批内差:2.3% |
样本量 | 20 μL |
检测时间 | 1 h 15 min |
检测仪器及波长 | 荧光酶标仪(激发光波长535 nm,发射光波长587 nm) |
保存条件 | 该产品可在-20°C避光条件下保存6个月。 |
有效期 | 6个月 |
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E-BC-K236-M | Aspartate Aminotransferase (AST/GOT) Activity Assay Kit | 96 T (43 samples) , 48 T (19 samples) |
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