alpha Tubulin Monoclonal Antibody

Price:
Size:
100 μL
25 μL
- 宿主: Mouse
- 反应性: Human;Mouse;E.coli;Scallop;Zebrafish
- 应用性: WB
Verified Samples |
Verified Samples in WB: HEK 293, CHO, Mouse Brain, Scallop Muscle, Zebrafish, E.coli, Rat Kidney, Scallop Muscle, Zebrafish, NIH/3T3, Raw264.7, Mouse Heart, Mouse Spleen, Mouse Brain, Mouse Kidney |
Dilution | WB 1:2000-5000 |
Clonality | Monoclonal |
Isotype | IgG2a |
Immunogen | Recombinant human TUBA protein |
Abbre | alpha Tubulin |
Synonyms | Alpha-tubulin 3, B alpha 1, FLJ25113, LIS3, TBA1A, TUBA1A, TUBA3, Tubulin B alp, Tubulin alpha 1A chain, Tubulin alpha 1a, Tubulin alpha 3, Tubulin alpha 3 chain, Tubulin alpha brain specific, Tubulin alpha-1A chain, Tubulin alpha-3 chain, Alpha tubulin 3 |
Swissprot | |
Calculated MW | 55 kDa |
Observed MW | 55 kDa |
Cellular Localization | Cytoskeleton, cytoplasmic microtubule, microtubule, microtubule cytoskeleton, Cytosol, Endosome, recycling endosome, Extracellular region or secreted, extracellular exosome, Nucleus, Other locations: cytoplasmic ribonucleoprotein granule, membrane raft, myelin sheath. |
Concentration | 1 mg/mL |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
Purification Method | Protein A/G Purification |
Clone No. | 2H2 |
Conjugation | Unconjugated |
Background | Tubulin is a highly conserved αβ dimeric protein present and is essential for all eukaryotes. αβ-Tubulin dimers assemble into microtubules, dynamic polymers important in a variety of functions, most noticeably, mitosis. Tubulin assembly and disassembly are linked to guanosine triphosphate hydrolysis. |
Western Blotting with anti-TUBA monoclonal antibody at dilution of 1:3000.
Lane 1: Scallop Muscle lysates, Lane 2: Zebrafish lysates
Western Blotting with anti-TUBA monoclonal antibody at dilution of 1:2000.
Lane 1: Hela cell lysates, Lane 2:jurkat cell lysates, Lane, 3: 293T lysates, Lane 4: MCF-7 cell lysates, Lane 5: HT-29 cell lysates, Lane 6:HepG2 cell lysates, Lane 7:Rat kidney lysates
Western Blotting with anti-TUBA monoclonal antibody at dilution of 1:2000.
Lane 1: NIH/3T3 cell lysates, Lane 2:Raw264.7 cell lysates, Lane 3: Mouse heart lysates, Lane 4: Mouse spleen lysates, Lane 5: Mouse brain lysates, Lane 6:Mouse kidney lysates
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