Avi-Tag Monoclonal Antibody

Price:
Size:
100 μL
25 μL
- 宿主: Mouse
- 反应性: All
- 应用性: WB
Verified Samples |
Verified Samples in WB: AVI-Tag fused recombinant protein (18kDa) |
Dilution | WB 1:3000-6000 |
Clonality | Monoclonal |
Isotype | IgG1 |
Immunogen | Synthetic peptide corresponding to AVI tag conjugated to keyhole limpet haemocyanin. |
Abbre | Avi-Tag |
Synonyms | Avi-Tag |
Calculated MW | 14 kDa |
Observed MW | 14 kDa |
Concentration | 1 mg/mL |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
Purification Method | Protein A/G Purification |
Clone No. | 1A3 |
Conjugation | Unconjugated |
Background | Avi tag is a biotin-acceptor peptide, GLNDIFEAQKIEWHE. Biotinylation of the Avi-tag is enzymatically added by the E. coli biotin ligase BirA. This results in a single biotin molecule covalently attached to the lysine residue within the Avi-tag |
Western blot of AVI-Tag fused recombinant protein with Anti-AVI mouse monoclonal antibody at 1:1000 dilution. Lane 1 : Recombinant AVI protein at 6.25ng; Lane 2 : Recombinant AVI protein at 12.5ng
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E-AB-1043 | Streptavidin(peroxidase/HRP conjugated) | 500 μL , 120 μL , 60 μL , 30 μL , 1 mL |
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E-IR-R304A | Western Blot Detection Kit | 50 Assays |
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