Caspase 3/7 Activity Assay Kit(Colorimetric Method)

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中文名称：Caspase 3/7活性检测试剂盒(分光光度法)

货号：E-CK-A383
文献（84）
说明书

说明书

Caspase 3/7 Activity Assay Kit(Colorimetric Method)

Price: ¥1800.00 ¥1000.00 ¥500.00

Size:

100 Assays 50 Assays 20 Assays

应用： 细胞凋亡-Caspase

细胞凋亡检测试剂盒全线5折

2025-08-19 ~ 2025-09-30

检测原理	Caspase 3也称CPP32、Yama或apopain，属于Caspase家族的CED-3亚家族(CED-3 subfamily)，是细胞凋亡过程中的一个关键酶，也是哺乳动物细胞中研究最多的一个caspase。Caspase 3在正常状态下以酶原的形式存在于胞浆中，没有活性；在细胞发生凋亡阶段，Caspase 3被激活，活化的caspase 3由两个大亚基和两个小亚基组成，裂解相应的胞浆胞核底物，最终导致细胞凋亡。本caspase 3/7活性检测试剂盒是基于caspase 3/7可以催化底物Ac-DEVD-pNA产生黄色的pNA(p-nitroaniline)，pNA在405nm附近有强吸收，从而可以通过测定吸光度来检测caspase 3/7的活性。
检测方法	比色法
样本类型	细胞;组织
检测时间	4-6 hours
检测仪器	分光光度计;酶标仪
自备试剂	PBS(E-BC-R187)，Bradford蛋白测定试剂盒(E-BC-K168-S或E-BC-K168-M)
保存温度	该产品可在-20°C避光条件下保存12个月。
有效期	12个月
运输条件	冰袋

pNA Standard Curve

Swim in Plastics: Clean Nanoplastics Cause Minimal Mortality but Alter Neurobehavioral and Molecular Rhythms in Fish

IF: 10.8

Journal： ENVIRONMENTAL SCIENCE & TECHNOLOGY (2025)

DOI：10.1021/acs.est.4c10984

Reactivity：Zebrafish

Sample Type：larvae tissue
MGST1 overexpression ameliorates mitochondrial dysfunction and ferroptosis during myocardial ischemia/reperfusion injury after heart transplantation

IF: 7.7

Journal： INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2025)

DOI：10.1016/j.ijbiomac.2025.140135

Reactivity：Mouse

Sample Type：heart graft tissue
Synthesis of iron oxide nanocrystals functionalized with hyaluronic acid and 131I-cetuximab for targeted combined (radio-photothermal) treatment of HepG2 cells

IF: 7.7

Journal： INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2025)

DOI：10.1016/j.ijbiomac.2025.140890

Reactivity：Human
NF-kappa-B inhibitor alpha mediates cancer stemness characteristics in oral squamous cell carcinoma by interacting with cathepsin B

IF: 7.7

Journal： INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2025)

DOI：10.1016/j.ijbiomac.2025.143690

Reactivity：Human
Carboxymethyl Poria cocos polysaccharides protect against septic kidney injury by regulating the Nrf2-NF-κB signaling pathway

IF: 7.7

Journal： INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2025)

DOI：10.1016/j.ijbiomac.2025.143030

Reactivity：Mouse

Sample Type：kidney tissue
Active Tumor Targeting by Core-Shell PDMS-HA Nanoparticles with Sequential Delivery of Doxorubicin and Quercetin to overcome P-glycoprotein efflux pump

IF: 5.8

Journal： Nanoscale (2025)

DOI：10.1039/D4NR03040K
Exploring the Internal Environmental Changes of Muscle Cells and Apoptotic Phase of Mitochondria in Dry-Cured Loin Using Electrical Stimulation: Promoting the Precise Regulation of Loin Ham Quality

IF: 5.7

Journal： JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2025)

DOI：10.1021/acs.jafc.4c09673

Reactivity：Pig

Sample Type：meat tissue
The Invertebrate-Derived Antimicrobial Peptide Cm-p5 Induces Cell Death and ROS Production in Melanoma Cells

IF: 5.4

Journal： Marine Drugs (2025)

DOI：10.3390/md23070273
Synergistic Anti-Cancer Activity of Melittin and Erlotinib in Non-Small Cell Lung Cancer

IF: 4.9

Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2025)

DOI：10.3390/ijms26072903

Reactivity：Human
Design, Synthesis, and Cytotoxicity Evaluation of Novel Indolin-2-One Based Molecules on Hepatocellular Carcinoma HepG2 Cells as Protein Kinase Inhibitors

IF: 4.2

Journal： MOLECULES (2025)

DOI：10.3390/molecules30051105

Reactivity：Human

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