Caspase 3/7 Activity Assay Kit(Colorimetric Method)

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中文名称：Caspase 3/7活性检测试剂盒(分光光度法)

货号：E-CK-A383
文献（84）
说明书

说明书

Caspase 3/7 Activity Assay Kit(Colorimetric Method)

Price: ¥1800.00 ¥1000.00 ¥500.00

Size:

100 Assays 50 Assays 20 Assays

应用： 细胞凋亡-Caspase

细胞凋亡检测试剂盒全线5折

2025-08-19 ~ 2025-09-30

检测原理	Caspase 3也称CPP32、Yama或apopain，属于Caspase家族的CED-3亚家族(CED-3 subfamily)，是细胞凋亡过程中的一个关键酶，也是哺乳动物细胞中研究最多的一个caspase。Caspase 3在正常状态下以酶原的形式存在于胞浆中，没有活性；在细胞发生凋亡阶段，Caspase 3被激活，活化的caspase 3由两个大亚基和两个小亚基组成，裂解相应的胞浆胞核底物，最终导致细胞凋亡。本caspase 3/7活性检测试剂盒是基于caspase 3/7可以催化底物Ac-DEVD-pNA产生黄色的pNA(p-nitroaniline)，pNA在405nm附近有强吸收，从而可以通过测定吸光度来检测caspase 3/7的活性。
检测方法	比色法
样本类型	细胞;组织
检测时间	4-6 hours
检测仪器	分光光度计;酶标仪
自备试剂	PBS(E-BC-R187)，Bradford蛋白测定试剂盒(E-BC-K168-S或E-BC-K168-M)
保存温度	该产品可在-20°C避光条件下保存12个月。
有效期	12个月
运输条件	冰袋

pNA Standard Curve

BmNPV p35 regulates apoptosis in Bombyx mori via a novel target of interaction with the BmVDAC2-BmRACK1 complex

IF: 3.2

Journal： INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY (2024)

DOI：10.1016/j.ibmb.2024.104125
Efficacy and Safety of Asparagusic Acid against Echinococcus multilocularis In Vitro and in a Murine Infection Model

IF: 2.8

Journal： Tropical Medicine and Infectious Disease (2024)

DOI：10.3390/tropicalmed9050110
Apelin?13 reduces high glucose?induced mitochondrial dysfunction in cochlear hair cells by inhibiting endoplasmic reticulum stress

IF: 2.7

Journal： Experimental and Therapeutic Medicine (2024)

DOI：10.3892/etm.2024.12515

PMID：38596659
ABRACL upregulated by transcription factor CBX4 promotes proliferation and migration and inhibits the apoptosis of gastric cancer cells

IF: 2.5

Journal： HISTOLOGY AND HISTOPATHOLOGY (2024)

DOI：10.14670/HH-18-812

Reactivity：Human
Effect of anti-inflammatory molecules from food on organoids derived from adenomatous polyps of FAP subjects

IF: 2.0

Journal： Tumori Journal (2024)

DOI：10.1177/03008916241291301
Analysis of the Anticancer Mechanism of OR3 Pigment from Streptomyces coelicolor JUACT03 Against the Human Hepatoma Cell Line Using a Proteomic Approach

IF: 1.8

Journal： CELL BIOCHEMISTRY AND BIOPHYSICS (2024)

DOI：10.1007/s12013-024-01258-0
Sensitivity of Primary Human Glioblastoma Cell Lines to the Mumps Virus Vaccine Strain

IF: 1.5

Journal： MOLECULAR BIOLOGY (2024)

DOI：10.1134/S0026893324700262

Reactivity：Human
Dictamnine Reduces Gestational Diabetic Complications in Streptozotocin-Induced Diabetic Pregnant Rats via Regulating Inflammation and NOX-2, MCP-1/AGE–RAGE Pathway

IF: 1.4

Journal： Revista Brasileira de Farmacognosia-Brazilian Journal of Pharmacognosy (2024)

DOI：10.1007/s43450-024-00591-y

Reactivity：Rat

Sample Type：pancreatic tissue
V2C-Driven Nanodelivery Platform Potentiates Synergistic Breast Cancer Therapy

IF: 11.4

Journal： ACS Materials Letters (2023)

DOI：10.1021/acsmaterialslett.3c00965

Reactivity：Human
Combined Donepezil with Astaxanthin via Nanostructured Lipid Carriers Effective Delivery to Brain for Alzheimer’s Disease in Rat Model

IF: 8.0

Journal： International Journal of Nanomedicine (2023)

DOI：10.2147/IJN.S417928

PMID：37534058

Reactivity：Rat

Sample Type：cortical tissue,hippocampal tissue

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