Caspase 3/7 Activity Assay Kit(Colorimetric Method)

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中文名称：Caspase 3/7活性检测试剂盒(分光光度法)

货号：E-CK-A383
文献（84）
说明书

说明书

Caspase 3/7 Activity Assay Kit(Colorimetric Method)

Price: ¥1800.00 ¥1000.00 ¥500.00

Size:

100 Assays 50 Assays 20 Assays

应用： 细胞凋亡-Caspase

细胞凋亡检测试剂盒全线5折

2025-08-19 ~ 2025-09-30

检测原理	Caspase 3也称CPP32、Yama或apopain，属于Caspase家族的CED-3亚家族(CED-3 subfamily)，是细胞凋亡过程中的一个关键酶，也是哺乳动物细胞中研究最多的一个caspase。Caspase 3在正常状态下以酶原的形式存在于胞浆中，没有活性；在细胞发生凋亡阶段，Caspase 3被激活，活化的caspase 3由两个大亚基和两个小亚基组成，裂解相应的胞浆胞核底物，最终导致细胞凋亡。本caspase 3/7活性检测试剂盒是基于caspase 3/7可以催化底物Ac-DEVD-pNA产生黄色的pNA(p-nitroaniline)，pNA在405nm附近有强吸收，从而可以通过测定吸光度来检测caspase 3/7的活性。
检测方法	比色法
样本类型	细胞;组织
检测时间	4-6 hours
检测仪器	分光光度计;酶标仪
自备试剂	PBS(E-BC-R187)，Bradford蛋白测定试剂盒(E-BC-K168-S或E-BC-K168-M)
保存温度	该产品可在-20°C避光条件下保存12个月。
有效期	12个月
运输条件	冰袋

pNA Standard Curve

Daidzein Ameliorates Cerebral Ischemic-reperfusion Induced Neuroinflammation in Wistar Rats via Inhibiting NF-κB Signaling Pathway

IF: 0.700

Journal： Pharmacognosy Magazine (2023)

DOI：10.1177/09731296221137378

Reactivity：Rat

Sample Type：brain tissue
TRIM8 regulates contrast induced renal cell injury via the TLR4/MyD88/ NF kB pathway

IF: 0.600

Journal： TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH (2023)

DOI：10.4314/tjpr.v22i1.4
Tin oxide nanoparticles trigger the formation of amyloid β oligomers/protofibrils and underlying neurotoxicity as a marker of Alzheimer's diseases

IF: 8.025

Journal： INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2022)

DOI：10.1016/j.ijbiomac.2022.01.190

PMID：35124024

Reactivity：Rat
Surface-Modified Polypyrrole-Coated PLCL and PLGA Nerve Guide Conduits Fabricated by 3D Printing and Electrospinning

IF: 6.978

Journal： BIOMACROMOLECULES (2022)

DOI：10.1021/acs.biomac.2c00626

PMID：36169096
Methyl salicylate retards mitochondria-mediated programmed cell death in peel spotting of ‘Sucrier’ banana during storage

IF: 6.751

Journal： POSTHARVEST BIOLOGY AND TECHNOLOGY (2022)

DOI：10.1016/j.postharvbio.2022.112099
Safety and Efficacy of Dietary Epigallocatechin Gallate Supplementation in Attenuating Hypertension via Its Modulatory Activities on the Intrarenal Renin–Angiotensin System in Spontaneously Hypertensive Rats

IF: 6.706

Journal： Nutrients (2022)

DOI：10.3390/nu14214605

PMID：36364864
Exploring the interaction of sesamol as an antilung cancer compound with albumin through spectroscopic and bioinformatic analyses and the mechanism of anticancer effect

IF: 6.212

Journal： Arabian Journal of Chemistry (2022)

DOI：10.1016/j.arabjc.2022.103941
In vitro and in vivo anti-tumor activity of Antrodia salmonea against twist-overexpressing HNSCC cells: Induction of ROS-mediated autophagic and apoptotic cell death

IF: 5.572

Journal： FOOD AND CHEMICAL TOXICOLOGY (2022)

DOI：10.1016/j.fct.2022.113564

PMID：36563924
Co-delivery of Docetaxel and Resveratrol by liposomes synergistically boosts antitumor efficiency against prostate cancer

IF: 5.112

Journal： EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES (2022)

DOI：10.1016/j.ejps.2022.106199

PMID：35533965
Zinc and selenium mitigated heavy metals mixture (Pb, Al, Hg and Mn) mediated hepatic-nephropathy via modulation of oxido-inflammatory status and NF?kB signaling in female albino rats

IF: 4.571

Journal： TOXICOLOGY (2022)

DOI：10.1016/j.tox.2022.153350

PMID：36220500

Reactivity：Rat

Sample Type：liver, kidney

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