Caspase 9 Activity Assay Kit(Colorimetric Method)
中文名称:Caspase 9活性检测试剂盒(分光光度法)

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- 应用: 细胞凋亡-Caspase
检测原理 | Caspase 9又称ICE-LAP6 或Mch6,是细胞凋亡信号转导过程中比较上游的一个caspase。线粒体释放细胞色素c以后,Caspase 9可以和细胞色素c以及Apaf1形成复合物,同时被激活。激活的caspase 9可以激活细胞凋亡的最关键酶caspase 3,从而促进后续的细胞凋亡信号。Caspase 9的激活可以通过磷酸化进行调控。本caspase 9活性检测试剂盒是基于caspase 9可以催化底物Ac-LEHD-pNA产生黄色的pNA(p-nitroaniline),pNA在405nm附近有强吸收,从而可以通过测定吸光度来检测caspase 9的活性。 |
检测方法 | 比色法 |
样本类型 | 细胞;组织 |
检测时间 | 4-6 hours |
检测仪器 | 分光光度计;酶标仪 |
自备试剂 | PBS(E-BC-R187),Bradford蛋白测定试剂盒(E-BC-K168-S或E-BC-K168-M) |
保存温度 | 该产品可在-20°C避光条件下保存12个月。 |
有效期 | 12个月 |
运输条件 | 冰袋 |
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