Catalase (CAT) Activity Assay Kit
中文名称:过氧化氢酶(CAT)比色法测试盒

Price:
Size:
96 T (40 samples)
48 T (16 samples)
- 样本类型: 血清(浆);动植物组织;细胞;培养液
- 检测仪器及波长: 酶标仪(400-410 nm,最佳检测波长为405 nm)
- 研究领域: 氧化应激
为了简化计算过程,可下载该产品计算文件。如使用中有任何问题,请随时联系技术支持
该指标在测定组织或细胞样本时,需要测定样本的总蛋白含量,推荐使用BCA法测蛋白,货号E-BC-K318-M
说明书或网站上未涉及的样本类型请先跟技术支持沟通,可进行售前验证
根据需测样本数,试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量,请点击按钮 计算
检测原理 |
过氧化氢酶催化H2O2分解为氧和水,钼酸铵能够迅速中止CAT分解H2O2的反应,而剩余的H2O2与钼酸铵作用产生一种淡黄色的络合物,在波长405 nm处测定其生成量,可计算出CAT的活力。
|
指标简称 | CAT |
灵敏度 | 1.12 U/mL |
检测范围 | 1.12-150 U/mL |
精密度 | 批间差:7.7% | 批内差:3.9% |
样本量 | 40 μL |
检测时间 | 40 min |
检测仪器及波长 | 酶标仪(400-410 nm,最佳检测波长为405 nm) |
保存条件 | 该产品可在 2-8°C避光条件下保存6个月。 |
有效期 | 6个月 |
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