Cell Mitochondrial Complex Ⅲ (Coenzyme Q-Cytochrome C Reductase) Activity Assay Kit
中文名称:细胞线粒体呼吸链复合物Ⅲ(辅酶Q-细胞色素C还原酶)比色法测试盒

Price:
Size:
96 T (94 samples)
48 T (46 samples)
- 样本类型: 细胞
- 检测仪器及波长: 酶标仪(540-560 nm,最佳检测波长为550 nm)
- 研究领域: 线粒体功能;能量代谢
该指标在测定组织或细胞样本时,需要测定样本的总蛋白含量,推荐使用BCA法测蛋白,货号E-BC-K318-M
说明书或网站上未涉及的样本类型请先跟技术支持沟通,可进行售前验证
根据需测样本数,试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量,请点击按钮 计算
检测原理 |
线粒体作为细胞器,是细胞内的“动力工厂”,是细胞有氧呼吸的主要场所,它的功能是通过氧化磷酸化进行能量转换,为细胞活动提供能量。其中,氧化过程由线粒体内膜上的4个呼吸链膜蛋白复合物(简称复合物Ⅰ、Ⅱ、Ⅲ和Ⅳ)来完成。线粒体呼吸链复合物Ⅲ又称为细胞色素c还原酶复合体,主要功能是将线粒体呼吸链复合物Ⅰ、Ⅱ形成的还原型辅酶Q10,氧化成氧化性辅酶Q10。线粒体呼吸链复合物Ⅲ将氧化型细胞色素c转化成还原型细胞色素c,造成550 nm吸光度上升,通过检测550 nm波长下吸光度上升的速率来计算酶活。
|
灵敏度 | 0.73 U/L |
检测范围 | 0.73-29.3 U/L |
精密度 | 批间差:4.5-10.0% | 批内差:3.5-5.0% |
样本量 | 40 μL(cell homogenate) |
检测时间 | 30 min |
检测仪器及波长 | 酶标仪(540-560 nm,最佳检测波长为550 nm) |
保存条件 | 该产品可在-20°C避光条件下保存3个月。 |
有效期 | 3个月 |
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