Cell Mitochondrial Complex Ⅴ (F0F1-ATPase/ATP Synthase) Activity Assay Kit
中文名称:细胞线粒体呼吸链复合物Ⅴ(F0F1-ATP酶/ATP合成酶)比色法测试盒

Price:
Size:
96 T (94 samples)
48 T (46 samples)
- 样本类型: 细胞
- 检测仪器及波长: 酶标仪(330-350 nm,最佳检测波长为340 nm)
- 研究领域: 线粒体功能;能量代谢
该指标在测定组织或细胞样本时,需要测定样本的总蛋白含量,推荐使用BCA法测蛋白,货号E-BC-K318-M
说明书或网站上未涉及的样本类型请先跟技术支持沟通,可进行售前验证
根据需测样本数,试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量,请点击按钮 计算
检测原理 |
线粒体复合物V又称为F0F1-ATP合成酶,ATP被F0F1-ATP合成酶水解后生成ADP,ADP在酶转化反应后,使还原型辅酶Ⅰ(NADH)转化为氧化型辅酶Ⅰ(NAD),通过检测340 nm处吸光度的变化速率,来计算F0F1-ATP酶活。
|
灵敏度 | 3.40 U/L |
检测范围 | 3.40-272.07 U/L |
精密度 | 批间差:3.0-6.5% | 批内差:3.0-4.0% |
样本量 | 20 μL(cell homogenate) |
检测时间 | 30 min |
检测仪器及波长 | 酶标仪(330-350 nm,最佳检测波长为340 nm) |
保存条件 | 该产品可在-20°C避光条件下保存6个月。 |
有效期 | 6个月 |
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