Cell Staining Buffer
中文名称：细胞染色缓冲液
Price:
| 保存温度 | 该产品可在 2~8°C条件下保存12个月。 |
| 有效期 | 12个月 |
| 运输条件 | 冰袋 |
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NAD+ Metabolism Reprogramming Drives SIRT1-Dependent Deacetylation Inducing PD-L1 Nuclear Localization in Cervical Cancer
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Metal-organic framework nanoparticles activate cGAS-STING pathway to improve radiotherapy sensitivity
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Microneedle-mediated hypoxic extracellular vesicle-encapsulated selenium nanoparticles delivery to treat androgenetic alopecia
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Multi-omics analysis reveals immunosuppression in oesophageal squamous cell carcinoma induced by creatine accumulation and HK3 deficiency
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Tremella polysaccharide microneedles loaded with magnetic dental pulp stem cell intracellular vesicles used for androgenic alopecia
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Promoting the transition from pyroptosis to apoptosis in endothelial cells: a novel approach to alleviate methylglyoxal-induced vascular damage
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Dietary Fiber Intake Improves Osteoporosis Caused by Chronic Lead Exposure by Restoring the Gut–Bone Axis
IF: 4.8Journal： Nutrients (2025) -
Cucurbitacin B induces oral squamous cell carcinomapyroptosis via GSDME and inhibits tumour growth
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Exosomes containing long non-coding RNA AGAP2-AS1 promote the differentiation of CD4+ T cells through the miR-424-5p/SGK1 axis in psoriasis
IF: 2.8Journal： Frontiers in Genetics (2025)
Q1:细胞染色缓冲液有什么用，我可以用PBS代替吗？一个样本需要多少细胞染色缓冲液？
细胞染色缓冲液主要作用可以保持细胞活力，使细胞变得稳定并且对损伤不太敏感；使pH敏感荧光染料产生的荧光信号强度最大化；减少抗体与靶细胞的非特异性结合；螯合金属阳离子，减少细胞之间的粘连。可以用1%BSA的PBS溶液替代。一个样本大概需要1.5 mL细胞染色缓冲液。
Q2:我的样本是小鼠的外周血和肺泡灌洗液，我取出之后怎么样能最大时间的保存样本？
流式实验建议用新鲜细胞做。如果您这边情况特殊，只能建议您先将制备好的单细胞悬液用4%多聚甲醛固定30min，然后用PBS洗涤两遍，保存在细胞染色缓冲液（可以用1 %BSA的PBS替代）中，置于4°C保存，试剂收到后尽快检测。
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