DNA-RNA Hybrid Monoclonal Antibody[S9.6]
![DNA-RNA Hybrid Monoclonal Antibody[S9.6]](http://files.elabscience.cn/assets/images/loading.png)
Price:
Size:
100 μL
25 μL
- 宿主： Mouse
- 反应性： All
- 应用性： IF;ChIP
| Verified Samples |
Verified Samples in IF: HeLa Verified Samples in ChIP: HeLa |
| Dilution | IF 1:200, ChIP 1:50-500, |
| Isotype | IgG2a |
| Immunogen | ΦX174 bacteriophage-derived synthetic DNA/RNA |
| Abbre | DNA-RNA Hybrid |
| Synonyms | DNA-RNA hybrid, RNA/DNA hybrid |
| Concentration | 1 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Protein A/G Purification |
| Clone No. | S9.6 |
| Conjugation | Unconjugated |
| Background | The DNA-RNA hybrids are a natural occurrence within eukaryotic cells and their level are high at sites of high transcriptional activity. They are non-canonical nucleic acid structures with transcriptional regulatory functions. Their presence is reported to predispose a locus to chromosomal breakage. The S9.6 monoclonal antibody recognizes DNA-RNA hybrids (also known as R-loops) and does not bind to single or double stranded DNA. The antibody has high affinity for DNA-RNA hybrids but also binds RNA-RNA hybrids that are AU-rich. The specificity of the antibody appears to be determined by a combination of sequence and structural dependency since R-loop sequence affects binding affinity. |
Immunofluorescentanalysis of (4% PFA) fixed HeLa cells using DNA-RNA hybrid mouse monoclonal antibody [S9.6] at dilution of 1:500.
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using anti-DNA-RNA hybrid Mouse Monoclonal Antibody [S9.6] at dilution of 1:500.
Chromatin immunoprecipitation analysis of HeLa cells genomic DNA(gDNA) using DNA-RNA hybrid Mouse Monoclonal Antibody [S9.6] at dilution of 1:200.
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