GST-Tag Monoclonal Antibody
Price:
Size:
100 μL
25 μL
- 宿主： Mouse
- 反应性： All
- 应用性： WB
| Verified Samples |
Verified Samples in WB: GST-Tag fused recombinant proteins |
| Dilution | WB 1:500-1000 |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Immunogen | Recombinant GST protein expressed by E.coli |
| Abbre | GST-Tag |
| Synonyms | GST, Glutathione S-transferase class-mu 26 kDa isozyme, Sj26 antigen, SjGST, Glutathione S Transferase |
| Calculated MW | 26 kDa |
| Observed MW | 26 kDa |
| Concentration | 1 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Protein A/G Purification |
| Clone No. | 4H3 |
| Conjugation | Unconjugated |
| Background | GST , Glutathione-S-transferase, a 26 kDa sequence of 211 amino acids, is another widely used affinity tag that increases solubility of the desired protein. GST tag has affinity for immobilized glutathione and is used for prokaryotic expression more frequently. It can be fused to either the N-terminus or C-terminus of a protein. |
Western blot of GST-Tag fused recombinant proteins with Anti-GST mouse monoclonalantibody at dilution of 1:1000. Lane 1 : Recombinant GST protein at 6.25ng; Lane 2 : Recombinant GST protein at 12.5ng; Lane 3: Recombinant GST protein at 25ng; Lane 4: Recombinant GST protein at 50ng
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