His-Tag Monoclonal Antibody
Price:
Size:
100 μL
25 μL
- 宿主： Mouse
- 反应性： All
- 应用性： WB;IP;FCM
| Verified Samples |
Verified Samples in WB: His-Tag fused recombinant protein Verified Samples in IP: 293F Verified Samples in FCM: CHO |
| Dilution | WB 1:1000-2000, IP 3μg/sample, FCM 0.2μg |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Immunogen | Synthetic peptide corresponding to 6His tag conjugated to keyhole limpet haemocyanin. |
| Abbre | His-Tag |
| Synonyms | HHHHHH epitope tag, HHHHHH tag, Hexa His tag, His tag, Polyhistidine Tag, 6 His epitope tag |
| Concentration | 1 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS, pH 7.2. Contains 0.05% proclin 300. |
| Purification Method | Protein A/G Purification |
| Clone No. | 1A5 |
| Conjugation | Unconjugated |
| Background | The 6xHis tag, also known as polyhistidine tag, His6 tag and/or hexa histidine tag, is an amino acid motif consisting of at least 6 histidine residues fused to the carboxyl (C-) or amino (N-) terminus of a target protein in transfected cells. It is one of the simplest and most widely used purification tags in the world. |
Western blot of His-Tag fused recombinant protein with Anti-His mouse monoclonal antibody at dilution of 1:1000.
IP Result of 293F cells transfected with His-Tag fusion protein,using anti-His-Tag mouse monoclonal antibody . Lane 1:input, Lane 2: anti-His-Tag mouse monoclonal antibody, Lane 3: mouse IgG1 Isotype Control
1x10^6 CHO cells Transfected with a His plasmid were stained with 0.2ug Anti-His tag mAb(E-AB-48003) and AF647 conjugated Goat Anti-mouse IgG (H+L);lsotype Control stained with 0.2ug mouse IgG and AF647 conjugated Goat Anti-mouse IgG (H+L)
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| E-AB-1076 | Goat Anti-Mouse IgG(H+L)(Elab Fluor® 647 conjugated) | 500 μL , 120 μL , 60 μL , 30 μL , 1 mL |
| E-AB-1087 | Elab Fluor® Violet 450-Goat Anti-Mouse IgG(H+L) | 500 μL , 120 μL , 1 mL |
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