Lipid Peroxide (LPO) Fluorometric Assay Kit
中文名称:脂质过氧化物(LPO)荧光法测试盒

Price:
Size:
96 T
- 样本类型: 细胞
- 检测仪器及波长: 荧光酶标仪(Ex/Em=500 nm/540 nm)、流式细胞仪(FITC)、荧光显微镜(FITC或GFP)
- 研究领域: 铁死亡
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根据需测样本数,试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量,请点击按钮 计算
检测原理 |
脂质过氧化物(LPO)是不饱和脂肪酸经过活性氧的作用所生成的物质。生成的LPO会继续氧化脂质体,从而在连锁反应中不断积累LPO。过量的LPO会分解成Acrolein, Malondialdehyde (MDA),4-hydroxy-2-nonenal (4-NHE)等具有高活性的醛类物质,对细胞产生毒性进而造成细胞死亡。
本试剂盒提供一种可以检测LPO形成的荧光探针C11-BODIPY 581/591。这种荧光探针可以与脂质过氧化途径中的脂质自由基等发生反应,从而起到检测细胞内脂质过氧化水平的作用。该荧光探针在正常情况下通过光激发发出红色荧光,随着脂质过氧化的过程荧光会从红色变为绿色。通过绿色荧光强度的增强,可以高灵敏度的检测LPO的形成。 |
指标简称 | LPO |
检测仪器及波长 | 荧光酶标仪(Ex/Em=500 nm/540 nm)、流式细胞仪(FITC)、荧光显微镜(FITC或GFP) |
保存条件 | 该产品可在-20°C避光条件下保存3个月。 |
有效期 | 3个月 |
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