Nitric Oxide (NO) Colorimetric Assay Kit (Nitrate Reductase Method)

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中文名称：一氧化氮（NO）比色法测试盒（硝酸还原酶法）

货号：E-BC-K135-M
文献（67）
说明书

说明书

Nitric Oxide (NO) Colorimetric Assay Kit (Nitrate Reductase Method)

Price: ¥980.00 ¥618.00

Size:

96 T (80 samples) 48 T (32 samples)

样本类型： 血清（浆）;尿液;组织;细胞;细胞上清
检测仪器及波长： 酶标仪（520-550 nm，最佳检测波长为530 nm）
研究领域： 氧化应激

为了简化计算过程，可下载该产品计算文件。如使用中有任何问题，请随时联系技术支持

该指标在测定组织或细胞样本时，需要测定样本的总蛋白含量，推荐使用BCA法测蛋白，货号E-BC-K318-M

说明书或网站上未涉及的样本类型请先跟技术支持沟通，可进行售前验证

根据需测样本数，试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量，请点击按钮计算

检测原理	一氧化氮（NO）化学性质活泼，在体内很快代谢为NO^2-和NO^3-，而NO^2-又进一步转化为NO^3-，本法利用硝酸还原酶特异性将NO^3-还原为NO^2-，通过显色深浅来测定其浓度的高低。
指标简称	NO
灵敏度	1.38 μmol/L
检测范围	1.38-40 μmol/L
精密度	批间差：8% \| 批内差：6%
样本量	80-200 μL
检测时间	1 h 40 min
检测仪器及波长	酶标仪（520-550 nm，最佳检测波长为530 nm）
保存条件	该产品可在-20°C避光条件下保存6个月。
有效期	6个月

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