Nitric Oxide (NO) Colorimetric Assay Kit (Nitrate Reductase Method)
中文名称:一氧化氮(NO)比色法测试盒(硝酸还原酶法)

Price:
Size:
96 T (80 samples)
48 T (32 samples)
- 样本类型: 血清(浆);尿液;组织;细胞;细胞上清
- 检测仪器及波长: 酶标仪(520-550 nm,最佳检测波长为530 nm)
- 研究领域: 氧化应激
为了简化计算过程,可下载该产品计算文件。如使用中有任何问题,请随时联系技术支持
该指标在测定组织或细胞样本时,需要测定样本的总蛋白含量,推荐使用BCA法测蛋白,货号E-BC-K318-M
说明书或网站上未涉及的样本类型请先跟技术支持沟通,可进行售前验证
根据需测样本数,试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量,请点击按钮 计算
检测原理 |
一氧化氮(NO)化学性质活泼,在体内很快代谢为NO2-和NO3-,而NO2-又进一步转化为NO3-,本法利用硝酸还原酶特异性将NO3-还原为NO2-,通过显色深浅来测定其浓度的高低。
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指标简称 | NO |
灵敏度 | 1.38 μmol/L |
检测范围 | 1.38-40 μmol/L |
精密度 | 批间差:8% | 批内差:6% |
样本量 | 80-200 μL |
检测时间 | 1 h 40 min |
检测仪器及波长 | 酶标仪(520-550 nm,最佳检测波长为530 nm) |
保存条件 | 该产品可在-20°C避光条件下保存6个月。 |
有效期 | 6个月 |
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