One-step TUNEL In Situ Apoptosis Kit (Green, Elab Fluor® 488)

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中文名称：一步法TUNEL原位细胞凋亡检测试剂盒（绿色，Elab Fluor® 488）

货号：E-CK-A321
文献（74）
说明书

说明书

One-step TUNEL In Situ Apoptosis Kit (Green, Elab Fluor® 488)

Price: ¥3600.00 ¥2000.00 ¥1500.00

Size:

100 Assays 50 Assays 20 Assays

应用： 细胞凋亡-DNA片段化

细胞凋亡检测试剂盒全线5折

2025-08-19 ~ 2025-09-30

实验操作

检测原理	细胞在发生凋亡时，会激活一些特异性的DNA内切酶，这些内切酶会切断核小体间的基因组DNA。使得凋亡细胞的DNA被切割成180~200bp片段，在琼脂糖凝胶上通常以180~200bp的阶梯状迁移。TdT酶（脱氧核糖核酸末端转移酶）将标记的dUTP连接到断裂DNA暴露的3'-OH末端，通过在这些末端添加荧光dUTP的方式来标记晚期凋亡细胞，从而可以通过荧光显微镜进行检测。
检测方法	荧光法
样本类型	石蜡切片;冰冻切片;细胞爬片;细胞涂片
检测时间	3 hours
检测仪器	荧光显微镜
荧光	Elab Fluor®488
Ex/Em	495/519
Channel set	FITC
Filter set	FITC
自备试剂	4%多聚甲醛(E-IR-R113),0.2% Triton X-100(E-IR-R122),PBS(E-BC-R187),ddH2O,含抗荧光淬灭剂的封片液(E-IR-R119),二甲苯,无水乙醇
保存温度	该产品可在-20°C避光条件下保存12个月。
有效期	12个月
运输条件	冰袋

Paraffin embedded rat heart was treated with DNAse I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNAse I treated sample (green). The cells were counterstained with DAPI (blue).This photo was taken by confocal microscope.

Paraffin embedded rat brain was treated with DNAse I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNAse I treated sample (green). The cells were counterstained with DAPI (blue).

Paraffin embedded mouse colon was treated with DNAse I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNAse I treated sample (green). The cells were counterstained with DAPI (blue).

Fluorescence microscope analysis of camptothecin-induced apoptosis of Hela cells.

Mixed samples of normal mouse spleen cells and DNase I treated mouse spleen cells were stained.

Ultrasound?targeted microbubble destruction technology delivering β?klotho to the heart enhances FGF21 sensitivity and attenuates heart remodeling post?myocardial infarction

IF: 5.7

Journal： INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE (2024)

DOI：10.3892/ijmm.2024.5378
Ursolic acid improves necroptosis via STAT3 signaling in intestinal ischemia/reperfusion injury

IF: 4.8

Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2024)

DOI：10.1016/j.intimp.2024.112463
Pim-1 kinase protects the liver from ischemia reperfusion injury by regulating dynamics-related protein 1

IF: 4.6

Journal： iScience (2024)

DOI：10.1016/j.isci.2024.110280
Acute stress response in gill of Pacific white shrimp Litopenaeus vannamei to high alkalinity

IF: 4.5

Journal： AQUACULTURE (2024)

DOI：10.1016/j.aquaculture.2024.740766
Pretreated MSCs with IronQ Transplantation Attenuate Microglia Neuroinflammation via the cGAS-STING Signaling Pathway

IF: 4.5

Journal： Journal of Inflammation Research (2024)

DOI：10.2147/JIR.S449579

PMID：38504697
IL-17A enhances the inflammatory response of glaucoma through Act1/TRAF6/NF-κB pathway

IF: 4.4

Journal： NEUROCHEMISTRY INTERNATIONAL (2024)

DOI：10.1016/j.neuint.2024.105787
Erythrocyte membrane biomimetic EGCG nanoparticles attenuate renal injury induced by diquat through the NF-κB/NLRP3 inflammasome pathway

IF: 4.4

Journal： Frontiers in Pharmacology (2024)

DOI：10.3389/fphar.2024.1414918
Remote Ischemic Postconditioning-Mediated Neuroprotection against Stroke by Promoting Ketone Body-Induced Ferroptosis Inhibition

IF: 4.1

Journal： ACS Chemical Neuroscience (2024)

DOI：10.1021/acschemneuro.4c00014
Disulfiram downregulates ferredoxin 1 to maintain copper homeostasis and inhibit inflammation in cerebral ischemia/reperfusion injury

IF: 3.8

Journal： Scientific Reports (2024)

DOI：10.1038/s41598-024-64981-x
Acute stress response in hepatopancreas of Pacific white shrimp Litopenaeus vannamei to high alkalinity

IF: 3.7

Journal： Aquaculture Reports (2024)

DOI：10.1016/j.aqrep.2024.101981

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