One-step TUNEL In Situ Apoptosis Kit (Green, Elab Fluor® 488)

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中文名称：一步法TUNEL原位细胞凋亡检测试剂盒（绿色，Elab Fluor® 488）

货号：E-CK-A321
文献（74）
说明书

说明书

One-step TUNEL In Situ Apoptosis Kit (Green, Elab Fluor® 488)

Price: ¥3600.00 ¥2000.00 ¥1500.00

Size:

100 Assays 50 Assays 20 Assays

应用： 细胞凋亡-DNA片段化

细胞凋亡检测试剂盒全线5折

2025-08-19 ~ 2025-09-30

实验操作

检测原理	细胞在发生凋亡时，会激活一些特异性的DNA内切酶，这些内切酶会切断核小体间的基因组DNA。使得凋亡细胞的DNA被切割成180~200bp片段，在琼脂糖凝胶上通常以180~200bp的阶梯状迁移。TdT酶（脱氧核糖核酸末端转移酶）将标记的dUTP连接到断裂DNA暴露的3'-OH末端，通过在这些末端添加荧光dUTP的方式来标记晚期凋亡细胞，从而可以通过荧光显微镜进行检测。
检测方法	荧光法
样本类型	石蜡切片;冰冻切片;细胞爬片;细胞涂片
检测时间	3 hours
检测仪器	荧光显微镜
荧光	Elab Fluor®488
Ex/Em	495/519
Channel set	FITC
Filter set	FITC
自备试剂	4%多聚甲醛(E-IR-R113),0.2% Triton X-100(E-IR-R122),PBS(E-BC-R187),ddH2O,含抗荧光淬灭剂的封片液(E-IR-R119),二甲苯,无水乙醇
保存温度	该产品可在-20°C避光条件下保存12个月。
有效期	12个月
运输条件	冰袋

Paraffin embedded rat heart was treated with DNAse I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNAse I treated sample (green). The cells were counterstained with DAPI (blue).This photo was taken by confocal microscope.

Paraffin embedded rat brain was treated with DNAse I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNAse I treated sample (green). The cells were counterstained with DAPI (blue).

Paraffin embedded mouse colon was treated with DNAse I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNAse I treated sample (green). The cells were counterstained with DAPI (blue).

Fluorescence microscope analysis of camptothecin-induced apoptosis of Hela cells.

Mixed samples of normal mouse spleen cells and DNase I treated mouse spleen cells were stained.

Placental mesenchymal stem cells ameliorate NLRP3 inflammasome-induced ovarian insufficiency by modulating macrophage M2 polarization

IF: 4.000

Journal： Journal of Ovarian Research (2023)

DOI：10.1186/s13048-023-01136-y

PMID：36945010
Tilapia Skin Peptides Inhibit Apoptosis, Inflammation, and Oxidative Stress to Improve Dry Eye Disease In Vitro and In Vivo

IF: 4.0

Journal： JOURNAL OF FOOD BIOCHEMISTRY (2023)

DOI：10.1155/2023/6761792
Peroxiredoxin-6 Released by Astrocytes Contributes to Neuroapoptosis During Ischemia

IF: 3.300

Journal： NEUROSCIENCE (2023)

DOI：10.1016/j.neuroscience.2023.01.003

PMID：36642396
Exosome-shuttled MYCBPAP from bone marrow mesenchymal stem cells regulates synaptic remodeling and ameliorates ischemic stroke in rats

IF: 2.8

Journal： JOURNAL OF CHEMICAL NEUROANATOMY (2023)

DOI：10.1016/j.jchemneu.2023.102309

PMID：37423468
Simvastatin inhibits proliferation and promotes apoptosis of oral squamous cell carcinoma through KLF2 signal

IF: 2.4

Journal： Journal of Oral Biosciences (2023)

DOI：10.1016/j.job.2023.08.006

PMID：37625505
β-Sitosterol Inhibits The Proliferation of Endometrial Cells via Regulating Smad7-Mediated TGF-β/Smads Signaling Pathway

IF: 2.0

Journal： Cell Journal (2023)

DOI：10.22074/CELLJ.2023.1989631.1230

Reactivity：Mouse

Sample Type：endometrium tissue
AEBP1 exacerbates myocardial ischemia-reperfusion injury via inhibition of IκBα.

IF: 1.2

Journal： FOLIA MORPHOLOGICA (2023)

DOI：10.5603/fm.96874
The role of neutrophil elastase in aortic valve calcification

IF: 8.440

Journal： Journal of Translational Medicine (2022)

DOI：10.1186/s12967-022-03363-1

PMID：35397552
Transcriptional activation of Proteasome 26S non-ATPase subunit 7 by forkhead box P3 participates in gastric cancer cell proliferation and apoptosis

IF: 6.832

Journal： Bioengineered (2022)

DOI：10.1080/21655979.2021.2018097

PMID：35037550
Dihydromyricetin protects against Doxorubicin-induced cardiotoxicity through activation of AMPK/mTOR pathway

IF: 6.656

Journal： PHYTOMEDICINE (2022)

DOI：10.1016/j.phymed.2022.154027

PMID：35278898

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