One-step TUNEL In Situ Apoptosis Kit (Green, Elab Fluor® 488)

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中文名称：一步法TUNEL原位细胞凋亡检测试剂盒（绿色，Elab Fluor® 488）

货号：E-CK-A321
文献（74）
说明书

说明书

One-step TUNEL In Situ Apoptosis Kit (Green, Elab Fluor® 488)

Price: ¥3600.00 ¥2000.00 ¥1500.00

Size:

100 Assays 50 Assays 20 Assays

应用： 细胞凋亡-DNA片段化

细胞凋亡检测试剂盒全线5折

2025-08-19 ~ 2025-09-30

实验操作

检测原理	细胞在发生凋亡时，会激活一些特异性的DNA内切酶，这些内切酶会切断核小体间的基因组DNA。使得凋亡细胞的DNA被切割成180~200bp片段，在琼脂糖凝胶上通常以180~200bp的阶梯状迁移。TdT酶（脱氧核糖核酸末端转移酶）将标记的dUTP连接到断裂DNA暴露的3'-OH末端，通过在这些末端添加荧光dUTP的方式来标记晚期凋亡细胞，从而可以通过荧光显微镜进行检测。
检测方法	荧光法
样本类型	石蜡切片;冰冻切片;细胞爬片;细胞涂片
检测时间	3 hours
检测仪器	荧光显微镜
荧光	Elab Fluor®488
Ex/Em	495/519
Channel set	FITC
Filter set	FITC
自备试剂	4%多聚甲醛(E-IR-R113),0.2% Triton X-100(E-IR-R122),PBS(E-BC-R187),ddH2O,含抗荧光淬灭剂的封片液(E-IR-R119),二甲苯,无水乙醇
保存温度	该产品可在-20°C避光条件下保存12个月。
有效期	12个月
运输条件	冰袋

Paraffin embedded rat heart was treated with DNAse I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNAse I treated sample (green). The cells were counterstained with DAPI (blue).This photo was taken by confocal microscope.

Paraffin embedded rat brain was treated with DNAse I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNAse I treated sample (green). The cells were counterstained with DAPI (blue).

Paraffin embedded mouse colon was treated with DNAse I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNAse I treated sample (green). The cells were counterstained with DAPI (blue).

Fluorescence microscope analysis of camptothecin-induced apoptosis of Hela cells.

Mixed samples of normal mouse spleen cells and DNase I treated mouse spleen cells were stained.

Comparison of intratumor and local immune response between MV X-ray FLASH and conventional radiotherapies

IF: 4.739

Journal： Clinical and Translational Radiation Oncology (2022)

DOI：10.1016/j.ctro.2022.11.005

PMID：36425537
Gestational Exposure to Cyfluthrin through Endoplasmic Reticulum (ER) Stress—Mediated PERK Signaling Pathway Impairs Placental Development

IF: 4.472

Journal： Toxics (2022)

DOI：10.3390/toxics10120733

PMID：36548566
Andrographis paniculata improves growth and non-specific immunity of shrimp Litopenaeus vannamei, and protects it from Vibrio alginolyticus by reducing oxidative stress and apoptosis

IF: 3.605

Journal： DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY (2022)

DOI：10.1016/j.dci.2022.104542

PMID：36122733
The W9 peptide inhibits osteoclastogenesis and osteoclast activity by downregulating osteoclast autophagy and promoting osteoclast apoptosis

IF: 2.611

Journal： JOURNAL OF MOLECULAR HISTOLOGY (2021)

DOI：10.1007/s10735-021-10030-0

PMID：34664129

E-CK-A211	Annexin V-FITC/PI Apoptosis Kit	200 Assays , 100 Assays , 50 Assays , 20 Assays
E-CK-A301	Mitochondrial Membrane Potential Assay Kit (with JC-1)	100 Assays , 50 Assays , 20 Assays
E-CK-A362	Enhanced Cell Counting Kit 8 (WST-8/CCK8)	500 Tests , 100 Tests , 500 Tests × 20
E-CK-A320	One-step TUNEL In Situ Apoptosis Kit (Green, FITC)	100 Assays , 50 Assays , 20 Assays

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技术资源

• 巨噬细胞研究热点及解决方案：Raw264.7/BMDM/THP-1定向极化M1和M2秘籍

• 巨噬细胞研究热点及解决方案：巨噬细胞代谢研究攻略

• 巨噬细胞研究热点及解决方案：组织驻留巨噬细胞高效检测宝典！

• TUNEL检测细胞凋亡原则及经验总结

• 怎么选择合适的TUNEL 凋亡检测试剂盒

• TUNEL常见问题及解决方案

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One-step TUNEL In Situ Apoptosis Kit (Green, Elab Fluor® 488)

中文名称：一步法TUNEL原位细胞凋亡检测试剂盒（绿色，Elab Fluor® 488）

Comparison of intratumor and local immune response between MV X-ray FLASH and conventional radiotherapies

Gestational Exposure to Cyfluthrin through Endoplasmic Reticulum (ER) Stress—Mediated PERK Signaling Pathway Impairs Placental Development

Andrographis paniculata improves growth and non-specific immunity of shrimp Litopenaeus vannamei, and protects it from Vibrio alginolyticus by reducing oxidative stress and apoptosis

The W9 peptide inhibits osteoclastogenesis and osteoclast activity by downregulating osteoclast autophagy and promoting osteoclast apoptosis

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技术资源