One-step TUNEL In Situ Apoptosis Kit (Green, FITC)

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中文名称：一步法TUNEL原位细胞凋亡检测试剂盒（绿色，FITC）

货号：E-CK-A320
文献（307）
说明书

说明书

One-step TUNEL In Situ Apoptosis Kit (Green, FITC)

Price: ¥3600.00 ¥2000.00 ¥1500.00

Size:

100 Assays 50 Assays 20 Assays

应用： 细胞凋亡-DNA片段化

细胞凋亡检测试剂盒全线5折

2025-08-19 ~ 2025-09-30

实验操作

检测原理	细胞在发生凋亡时，会激活一些特异性的DNA内切酶，这些内切酶会切断核小体间的基因组DNA。使得凋亡细胞的DNA被切割成180~200bp片段，在琼脂糖凝胶上通常以180~200bp的阶梯状迁移。TdT酶（脱氧核糖核酸末端转移酶）将标记的dUTP连接到断裂DNA暴露的3'-OH末端，通过在这些末端添加荧光dUTP的方式来标记晚期凋亡细胞，从而可以通过荧光显微镜进行检测。
检测方法	荧光法
样本类型	石蜡切片;冰冻切片;细胞爬片;细胞涂片
检测时间	3 hours
检测仪器	荧光显微镜
荧光	FITC
Ex/Em	490/520
Channel set	FITC
Filter set	FITC
自备试剂	4%多聚甲醛(E-IR-R113),0.2% Triton X-100(E-IR-R122),PBS(E-BC-R187),ddH2O,含抗荧光淬灭剂的封片液(E-IR-R119),二甲苯,无水乙醇
保存温度	该产品可在-20°C避光条件下保存12个月。
有效期	12个月
运输条件	冰袋

Paraffin embedded rat heart was treated with DNase I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNase I treated sample (green). The cells were counterstained with DAPI (blue).This photo was taken by confocal microscope.

Paraffin embedded mouse liver was treated with DNase I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNase I treated sample (green). The cells were counterstained with DAPI (blue).This photo was taken by confocal microscope

Paraffin embedded mouse spleen was treated with DNase I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNase I treated sample (green). The cells were counterstained with DAPI (blue).This photo was taken by confocal microscop

Fluorescence microscope analysis of camptothecin-induced apoptosis of Hela cells.

Mixed samples of normal mouse spleen cells and DNase I treated mouse spleen cells were stained.

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DOI：10.1007/s10815-024-03327-y
Human amniotic mesenchymal stem cells-derived conditioned medium and exosomes alleviate oxidative stress-induced retinal degeneration by activating PI3K/Akt/FoxO3 pathway

IF: 3.0

Journal： EXPERIMENTAL EYE RESEARCH (2024)

DOI：10.1016/j.exer.2024.109919
Protective effects of curcumin on corneal endothelial cell PANoptosis and monocyte adhesion induced by tumor necrosis factor-alpha and interferon-gamma in rats

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DOI：10.1016/j.exer.2024.109952
Histopathological evaluation of the effects of Flattening Filter (FF) and Flattening Filter Free (FFF) X-ray: In vivo malignant melanoma cancer models

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Journal： RADIATION PHYSICS AND CHEMISTRY (2024)

DOI：10.1016/j.radphyschem.2024.111615
CaMKII Exacerbates Doxorubicin‐Induced Cardiotoxicity by Promoting Ubiquitination Through USP10 Inhibition

IF: 2.9

Journal： Cancer Medicine (2024)

DOI：10.1002/cam4.70286
Oxyberberine protects middle cerebral artery occlusion triggered cerebral injury through TLR4/NLRP3 pathway in rats

IF: 2.8

Journal： JOURNAL OF CHEMICAL NEUROANATOMY (2024)

DOI：10.1016/j.jchemneu.2024.102393

PMID：38246265
Acupuncture Extended the Thrombolysis Window by Suppressing Blood–Brain Barrier Disruption and Regulating Autophagy–Apoptosis Balance after Ischemic Stroke

IF: 2.7

Journal： Brain Sciences (2024)

DOI：10.3390/brainsci14040399
Diallyl trisulfide regulates PGK1/Nrf2 expression and reduces inflammation to alleviate neurological damage in mice after traumatic brain injury

IF: 2.7

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DOI：10.1016/j.brainres.2024.149116
Vitamin B6 ameliorates acute pancreatitis by suppressing the caspase3 signaling pathway

IF: 2.5

Journal： BMC GASTROENTEROLOGY (2024)

DOI：10.1186/s12876-024-03248-1

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