One-step TUNEL In Situ Apoptosis Kit (Green, FITC)

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中文名称：一步法TUNEL原位细胞凋亡检测试剂盒（绿色，FITC）

货号：E-CK-A320
文献（307）
说明书

说明书

One-step TUNEL In Situ Apoptosis Kit (Green, FITC)

Price: ¥3600.00 ¥2000.00 ¥1500.00

Size:

100 Assays 50 Assays 20 Assays

应用： 细胞凋亡-DNA片段化

细胞凋亡检测试剂盒全线5折

2025-08-19 ~ 2025-09-30

实验操作

检测原理	细胞在发生凋亡时，会激活一些特异性的DNA内切酶，这些内切酶会切断核小体间的基因组DNA。使得凋亡细胞的DNA被切割成180~200bp片段，在琼脂糖凝胶上通常以180~200bp的阶梯状迁移。TdT酶（脱氧核糖核酸末端转移酶）将标记的dUTP连接到断裂DNA暴露的3'-OH末端，通过在这些末端添加荧光dUTP的方式来标记晚期凋亡细胞，从而可以通过荧光显微镜进行检测。
检测方法	荧光法
样本类型	石蜡切片;冰冻切片;细胞爬片;细胞涂片
检测时间	3 hours
检测仪器	荧光显微镜
荧光	FITC
Ex/Em	490/520
Channel set	FITC
Filter set	FITC
自备试剂	4%多聚甲醛(E-IR-R113),0.2% Triton X-100(E-IR-R122),PBS(E-BC-R187),ddH2O,含抗荧光淬灭剂的封片液(E-IR-R119),二甲苯,无水乙醇
保存温度	该产品可在-20°C避光条件下保存12个月。
有效期	12个月
运输条件	冰袋

Paraffin embedded rat heart was treated with DNase I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNase I treated sample (green). The cells were counterstained with DAPI (blue).This photo was taken by confocal microscope.

Paraffin embedded mouse liver was treated with DNase I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNase I treated sample (green). The cells were counterstained with DAPI (blue).This photo was taken by confocal microscope

Paraffin embedded mouse spleen was treated with DNase I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNase I treated sample (green). The cells were counterstained with DAPI (blue).This photo was taken by confocal microscop

Fluorescence microscope analysis of camptothecin-induced apoptosis of Hela cells.

Mixed samples of normal mouse spleen cells and DNase I treated mouse spleen cells were stained.

EGFR/MAPK signaling pathway acts as a potential therapeutic target for sulforaphane-rescued heart tube malformation induced by various concentrations of PhIP exposure

IF: 7.9

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DOI：10.1016/j.phymed.2023.155270

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Ameliorative effect of anisodamine (654-1/654-2) against myocardial dysfunction induced by septic shock via the NF-κB/NLRP-3 or the PI3K-AKT/NF-κB pathway

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Journal： PHYTOMEDICINE (2023)

DOI：10.1016/j.phymed.2023.155277

PMID：38128396
Salvianolic acid A provides neuroprotective effects on cerebral ischemia-reperfusion injury in rats via PKA/CREB/c-Fos signaling pathway

IF: 7.9

Journal： PHYTOMEDICINE (2023)

DOI：10.1016/j.phymed.2023.155326

PMID：38185068
PM2.5 leads to adverse pregnancy outcomes by inducing trophoblast oxidative stress and mitochondrial apoptosis via KLF9/CYP1A1 transcriptional axis

IF: 7.7

Journal： eLife (2023)

DOI：10.7554/eLife.85944

PMID：37737576
Antimicrobial peptide cathelicidin LL-37 preserves intestinal barrier and organ function in rats with heat stroke

IF: 7.500

Journal： BIOMEDICINE & PHARMACOTHERAPY (2023)

DOI：10.1016/j.biopha.2023.114565

PMID：36958193
Angiotensin-(1–7) ameliorates sepsis-induced cardiomyopathy by alleviating inflammatory response and mitochondrial damage through the NF-κB and MAPK pathways

IF: 7.400

Journal： Journal of Translational Medicine (2023)

DOI：10.1186/s12967-022-03842-5

PMID：36593471
SIRT3 ameliorates diabetes-associated cognitive dysfunction via regulating mitochondria-associated ER membranes

IF: 7.4

Journal： Journal of Translational Medicine (2023)

DOI：10.1186/s12967-023-04246-9

PMID：37481555
Ozone fumigation promotes antioxidant activities to retard chlorophyll degradation and cell death in ‘Jinda’ chili during storage

IF: 7.000

Journal： POSTHARVEST BIOLOGY AND TECHNOLOGY (2023)

DOI：10.1016/j.postharvbio.2023.112375
Melatonin Alleviates Retinal Ischemia–Reperfusion Injury by Inhibiting p53–Mediated Ferroptosis

IF: 7.000

Journal： Antioxidants (2023)

DOI：10.3390/antiox12061173

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miR-30e-5p-mediated FOXD1 promotes cell proliferation by blocking cellular senescence and apoptosis through p21/CDK2/Rb signaling in head and neck carcinoma

IF: 7.0

Journal： Cell Death Discovery (2023)

DOI：10.1038/s41420-023-01571-2

PMID：37563111

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