One-step TUNEL In Situ Apoptosis Kit (Green, FITC)

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中文名称：一步法TUNEL原位细胞凋亡检测试剂盒（绿色，FITC）

货号：E-CK-A320
文献（307）
说明书

说明书

One-step TUNEL In Situ Apoptosis Kit (Green, FITC)

Price: ¥3600.00 ¥2000.00 ¥1500.00

Size:

100 Assays 50 Assays 20 Assays

应用： 细胞凋亡-DNA片段化

细胞凋亡检测试剂盒全线5折

2025-08-19 ~ 2025-09-30

实验操作

检测原理	细胞在发生凋亡时，会激活一些特异性的DNA内切酶，这些内切酶会切断核小体间的基因组DNA。使得凋亡细胞的DNA被切割成180~200bp片段，在琼脂糖凝胶上通常以180~200bp的阶梯状迁移。TdT酶（脱氧核糖核酸末端转移酶）将标记的dUTP连接到断裂DNA暴露的3'-OH末端，通过在这些末端添加荧光dUTP的方式来标记晚期凋亡细胞，从而可以通过荧光显微镜进行检测。
检测方法	荧光法
样本类型	石蜡切片;冰冻切片;细胞爬片;细胞涂片
检测时间	3 hours
检测仪器	荧光显微镜
荧光	FITC
Ex/Em	490/520
Channel set	FITC
Filter set	FITC
自备试剂	4%多聚甲醛(E-IR-R113),0.2% Triton X-100(E-IR-R122),PBS(E-BC-R187),ddH2O,含抗荧光淬灭剂的封片液(E-IR-R119),二甲苯,无水乙醇
保存温度	该产品可在-20°C避光条件下保存12个月。
有效期	12个月
运输条件	冰袋

Paraffin embedded rat heart was treated with DNase I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNase I treated sample (green). The cells were counterstained with DAPI (blue).This photo was taken by confocal microscope.

Paraffin embedded mouse liver was treated with DNase I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNase I treated sample (green). The cells were counterstained with DAPI (blue).This photo was taken by confocal microscope

Paraffin embedded mouse spleen was treated with DNase I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNase I treated sample (green). The cells were counterstained with DAPI (blue).This photo was taken by confocal microscop

Fluorescence microscope analysis of camptothecin-induced apoptosis of Hela cells.

Mixed samples of normal mouse spleen cells and DNase I treated mouse spleen cells were stained.

PM2.5 induce myocardial injury in hyperlipidemic mice through ROS-pyroptosis signaling pathway

IF: 6.800

Journal： ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2023)

DOI：10.1016/j.ecoenv.2023.114699

PMID：36889212
Triphenyl phosphate induced apoptosis of mice testicular Leydig cells and TM3 cells through ROS-mediated mitochondrial fusion inhibition

IF: 6.800

Journal： ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2023)

DOI：10.1016/j.ecoenv.2023.114876

PMID：37027944
Toxic effects of bisphenol S on mice heart and human umbilical cord endothelial cells

IF: 6.8

Journal： ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2023)

DOI：10.1016/j.ecoenv.2023.115652

PMID：37924801
circFLNA promotes intestinal injury during abdominal sepsis through Fas-mediated apoptosis pathway by sponging miR-766-3p

IF: 6.700

Journal： INFLAMMATION RESEARCH (2023)

DOI：10.1007/s00011-023-01688-1

PMID：36625877
Acupuncture regulates the apoptosis of ovarian granulosa cells in polycystic ovarian syndrome-related abnormal follicular development through LncMEG3-mediated inhibition of miR-21-3p

IF: 6.7

Journal： BIOLOGICAL RESEARCH (2023)

DOI：10.1186/s40659-023-00441-6

PMID：37303036
CTRP12 ameliorates post-myocardial infarction heart failure through down-regulation of cardiac apoptosis, oxidative stress and inflammation by influencing the TAK1-p38 MAPK/JNK pathway

IF: 6.7

Journal： INFLAMMATION RESEARCH (2023)

DOI：10.1007/s00011-023-01758-4

PMID：37382682
Extracellular Vesicles from Mycoplasma gallisepticum: Modulators of Macrophage Activation and Virulence

IF: 6.4

Journal： JOURNAL OF INFECTIOUS DISEASES (2023)

DOI：10.1093/infdis/jiad486

PMID：37929888
Alfuzosin ameliorates diabetes by boosting PGK1 activity in diabetic mice

IF: 6.100

Journal： LIFE SCIENCES (2023)

DOI：10.1016/j.lfs.2023.121491

PMID：36758669

Reactivity：Mouse

Sample Type：Liver tissue
Diosmetin alleviates benzo[a]pyrene-exacerbated H1N1 influenza virus-induced acute lung injury and dysregulation of inflammation through modulation of the PPAR-γ-NF-κB/P38 MAPK signaling axis

IF: 6.100

Journal： Food & Function (2023)

DOI：10.1039/D2FO02590F

PMID：36942763
Cinnamaldehyde Alleviates Bone Loss by Targeting Oxidative Stress and Mitochondrial Damage via the Nrf2/HO-1 Pathway in BMSCs and Ovariectomized Mice

IF: 6.1

Journal： JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2023)

DOI：10.1021/acs.jafc.3c03501

PMID：37917162

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