One-step TUNEL In Situ Apoptosis Kit (Green, FITC)

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中文名称：一步法TUNEL原位细胞凋亡检测试剂盒（绿色，FITC）

货号：E-CK-A320
文献（307）
说明书

说明书

One-step TUNEL In Situ Apoptosis Kit (Green, FITC)

Price: ¥3600.00 ¥2000.00 ¥1500.00

Size:

100 Assays 50 Assays 20 Assays

应用： 细胞凋亡-DNA片段化

细胞凋亡检测试剂盒全线5折

2025-08-19 ~ 2025-09-30

实验操作

检测原理	细胞在发生凋亡时，会激活一些特异性的DNA内切酶，这些内切酶会切断核小体间的基因组DNA。使得凋亡细胞的DNA被切割成180~200bp片段，在琼脂糖凝胶上通常以180~200bp的阶梯状迁移。TdT酶（脱氧核糖核酸末端转移酶）将标记的dUTP连接到断裂DNA暴露的3'-OH末端，通过在这些末端添加荧光dUTP的方式来标记晚期凋亡细胞，从而可以通过荧光显微镜进行检测。
检测方法	荧光法
样本类型	石蜡切片;冰冻切片;细胞爬片;细胞涂片
检测时间	3 hours
检测仪器	荧光显微镜
荧光	FITC
Ex/Em	490/520
Channel set	FITC
Filter set	FITC
自备试剂	4%多聚甲醛(E-IR-R113),0.2% Triton X-100(E-IR-R122),PBS(E-BC-R187),ddH2O,含抗荧光淬灭剂的封片液(E-IR-R119),二甲苯,无水乙醇
保存温度	该产品可在-20°C避光条件下保存12个月。
有效期	12个月
运输条件	冰袋

Paraffin embedded rat heart was treated with DNase I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNase I treated sample (green). The cells were counterstained with DAPI (blue).This photo was taken by confocal microscope.

Paraffin embedded mouse liver was treated with DNase I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNase I treated sample (green). The cells were counterstained with DAPI (blue).This photo was taken by confocal microscope

Paraffin embedded mouse spleen was treated with DNase I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNase I treated sample (green). The cells were counterstained with DAPI (blue).This photo was taken by confocal microscop

Fluorescence microscope analysis of camptothecin-induced apoptosis of Hela cells.

Mixed samples of normal mouse spleen cells and DNase I treated mouse spleen cells were stained.

CX3CL1 represses autophagy via CX3CR1/ CaMKIIδ/HDAC4/Rubicon axis and exacerbates chronic intermittent hypoxia induced Kupffer cell apoptosis

IF: 4.8

Journal： CELLULAR SIGNALLING (2023)

DOI：10.1016/j.cellsig.2023.110873

PMID：37640194
Aerobic exercise mitigates hippocampal neuronal apoptosis by regulating DAPK1/CDKN2A/REDD1/FoXO1/FasL signaling pathway in D-galactose-induced aging mice

IF: 4.8

Journal： FASEB JOURNAL (2023)

DOI：10.1096/fj.202300847RR

PMID：37768886
Human embryonic stem cells exert antitumor effects on prostate cancer cells in a co-culture microenvironment

IF: 4.700

Journal： Frontiers in Oncology (2023)

DOI：10.3389/fonc.2023.1164250

PMID：37313467
An intersectional genetic approach for simultaneous cell type-specific labelling and gene knockout in the mouse

IF: 4.600

Journal： DEVELOPMENT (2023)

DOI：10.1242/dev.201198

PMID：36786332
The Large Molecular Weight Polysaccharide from Wild Cordyceps and Its Antitumor Activity on H22 Tumor-Bearing Mice

IF: 4.600

Journal： MOLECULES (2023)

DOI：10.3390/molecules28083351

PMID：37110586
Effects of the Supercritical Fluid Extract of Magnolia figo on Inducing the Apoptosis of Human Non-Small-Cell Lung Cancer Cells

IF: 4.6

Journal： MOLECULES (2023)

DOI：10.3390/molecules28217445

PMID：37959865
Molecular mechanism of Wilms tumor 1-associated protein in diabetes-related dry eye disease by mediating m6A methylation modification of lncRNA NEAT1

IF: 4.5

Journal： JOURNAL OF DRUG TARGETING (2023)

DOI：10.1080/1061186X.2023.2300682

PMID：38153328
Bone marrow-derived c-kit positive stem cell administration protects against diabetes-induced nephropathy in a rat model by reversing PI3K/AKT/GSK-3β pathway and inhibiting cell apoptosis

IF: 4.300

Journal： MOLECULAR AND CELLULAR BIOCHEMISTRY (2023)

DOI：10.1007/s11010-023-04750-y

PMID：37129768
Effects of fresh bone marrow mononuclear cell therapy in rat model of retinopathy of prematurity

IF: 4.3

Journal： Regenerative Therapy (2023)

DOI：10.1016/j.reth.2023.05.009

PMID：37334242
Vetting of New 2,5-Bis (2,2,2-trifluoroethoxy) Phenyl-Linked 1,3-Thiazolidine-4-one Derivatives as AURKA and VEGFR-2 Inhibitor Antiglioma Agents Assisted with In Vitro and In Silico Studies

IF: 4.1

Journal： ACS Omega (2023)

DOI：10.1021/acsomega.3c04662

PMID：38027362

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