One-step TUNEL In Situ Apoptosis Kit (Green, FITC)

一键复制产品信息

中文名称：一步法TUNEL原位细胞凋亡检测试剂盒（绿色，FITC）

货号：E-CK-A320
文献（307）
说明书

说明书

One-step TUNEL In Situ Apoptosis Kit (Green, FITC)

Price: ¥3600.00 ¥2000.00 ¥1500.00

Size:

100 Assays 50 Assays 20 Assays

应用： 细胞凋亡-DNA片段化

细胞凋亡检测试剂盒全线5折

2025-08-19 ~ 2025-09-30

实验操作

检测原理	细胞在发生凋亡时，会激活一些特异性的DNA内切酶，这些内切酶会切断核小体间的基因组DNA。使得凋亡细胞的DNA被切割成180~200bp片段，在琼脂糖凝胶上通常以180~200bp的阶梯状迁移。TdT酶（脱氧核糖核酸末端转移酶）将标记的dUTP连接到断裂DNA暴露的3'-OH末端，通过在这些末端添加荧光dUTP的方式来标记晚期凋亡细胞，从而可以通过荧光显微镜进行检测。
检测方法	荧光法
样本类型	石蜡切片;冰冻切片;细胞爬片;细胞涂片
检测时间	3 hours
检测仪器	荧光显微镜
荧光	FITC
Ex/Em	490/520
Channel set	FITC
Filter set	FITC
自备试剂	4%多聚甲醛(E-IR-R113),0.2% Triton X-100(E-IR-R122),PBS(E-BC-R187),ddH2O,含抗荧光淬灭剂的封片液(E-IR-R119),二甲苯,无水乙醇
保存温度	该产品可在-20°C避光条件下保存12个月。
有效期	12个月
运输条件	冰袋

Paraffin embedded rat heart was treated with DNase I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNase I treated sample (green). The cells were counterstained with DAPI (blue).This photo was taken by confocal microscope.

Paraffin embedded mouse liver was treated with DNase I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNase I treated sample (green). The cells were counterstained with DAPI (blue).This photo was taken by confocal microscope

Paraffin embedded mouse spleen was treated with DNase I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNase I treated sample (green). The cells were counterstained with DAPI (blue).This photo was taken by confocal microscop

Fluorescence microscope analysis of camptothecin-induced apoptosis of Hela cells.

Mixed samples of normal mouse spleen cells and DNase I treated mouse spleen cells were stained.

Chondroprotective effects of bone marrow mesenchymal stem cell-derived exosomes in osteoarthritis

IF: 3.0

Journal： JOURNAL OF BIOENERGETICS AND BIOMEMBRANES (2023)

DOI：10.1007/s10863-023-09991-6

PMID：38012335
Circ-PDZD8 promotes cell growth and glutamine metabolism in non-small cell lung cancer by enriching LARP1 via sequestering miR-330-5p

IF: 2.9

Journal： Thoracic Cancer (2023)

DOI：10.1111/1759-7714.15006

PMID：37349870
Sevoflurane Postconditioning Attenuates Cerebral Ischemia-Reperfusion Injury by Inhibiting SP1/ACSL4-Mediated Ferroptosis

IF: 2.800

Journal： HUMAN & EXPERIMENTAL TOXICOLOGY (2023)

DOI：10.1177/09603271231160477

PMID：36842993
Inhalation exposure to crude oil vapor induces behavioural deficits by increasing oxidative stress and histopathological changes in rat hippocampus: Quercetin therapeutic approach

IF: 2.800

Journal： JOURNAL OF CHEMICAL NEUROANATOMY (2023)

DOI：10.1016/j.jchemneu.2023.102290

PMID：37225059
Effect of dual optical pulses with temporal energy distribution on laser ablation performance in in vivo zebrafish model

IF: 2.8

Journal： Journal of Biophotonics (2023)

DOI：10.1002/jbio.202300232

PMID：37651611
CX3CL1/CX3CR1 axis alleviates inflammation and apoptosis in human nucleus pulpous cells via M2 macrophage polarization

IF: 2.7

Journal： Experimental and Therapeutic Medicine (2023)

DOI：10.3892/etm.2023.12058

PMID：37324510
Effects of pharmacological doses of niacin on subacute glucocorticoid-induced testicular damage in rats

IF: 2.6

Journal： Pharmacology Research & Perspectives (2023)

DOI：10.1002/prp2.1128

PMID：37589322
Targeting Autophagy With the Synergistic Combination of Chloroquine and Rapamycin as a Novel Effective Treatment for Well-differentiated Liposarcoma

IF: 2.5

Journal： Cancer Genomics & Proteomics (2023)

DOI：10.21873/cgp.20384

PMID：37400145
Protective effect of small extracellular vesicles (EVs) derived from ACE2-modified human umbilical cord mesenchymal stem cells against renal ischemia–reperfusion injury

IF: 2.5

Journal： NEPHROLOGY (2023)

DOI：10.1111/nep.14237

PMID：37667547
The effect of microRNA-663b in the inhibition of interleukin-1-induced nucleus pulposus cell apoptosis and inflammatory response

IF: 2.200

Journal： JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY (2023)

DOI：10.26402/jpp.2023.1.09

PMID：37245236

E-CK-A211	Annexin V-FITC/PI Apoptosis Kit	200 Assays , 100 Assays , 50 Assays , 20 Assays
E-CK-A301	Mitochondrial Membrane Potential Assay Kit (with JC-1)	100 Assays , 50 Assays , 20 Assays
E-CK-A362	Enhanced Cell Counting Kit 8 (WST-8/CCK8)	500 Tests , 100 Tests , 500 Tests × 20
E-CK-A321	One-step TUNEL In Situ Apoptosis Kit (Green, Elab Fluor® 488)	100 Assays , 50 Assays , 20 Assays