One-step TUNEL In Situ Apoptosis Kit (Red, Elab Fluor® 594)

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中文名称：一步法TUNEL原位细胞凋亡检测试剂盒（红色，Elab Fluor® 594）

货号：E-CK-A322
文献（61）
说明书

说明书

One-step TUNEL In Situ Apoptosis Kit (Red, Elab Fluor® 594)

Price: ¥3600.00 ¥2000.00 ¥1500.00

Size:

100 Assays 50 Assays 20 Assays

应用： 细胞凋亡-DNA片段化

细胞凋亡检测试剂盒全线5折

2025-08-19 ~ 2025-09-30

实验操作

检测原理	细胞在发生凋亡时，会激活一些特异性的DNA内切酶，这些内切酶会切断核小体间的基因组DNA。使得凋亡细胞的DNA被切割成180~200bp片段，在琼脂糖凝胶上通常以180~200bp的阶梯状迁移。TdT酶（脱氧核糖核酸末端转移酶）将标记的dUTP连接到断裂DNA暴露的3'-OH末端，通过在这些末端添加荧光dUTP的方式来标记晚期凋亡细胞，从而可以通过荧光显微镜进行检测。
检测方法	荧光法
样本类型	石蜡切片;冰冻切片;细胞爬片;细胞涂片
检测时间	3 hours
检测仪器	荧光显微镜
荧光	Elab Fluor®594
Ex/Em	590/617
Filter set	TRITC
自备试剂	4%多聚甲醛(E-IR-R113),0.2% Triton X-100(E-IR-R122),PBS(E-BC-R187),ddH2O,含抗荧光淬灭剂的封片液(E-IR-R119),二甲苯,无水乙醇
保存温度	该产品可在-20°C避光条件下保存12个月。
有效期	12个月
运输条件	冰袋

Paraffin embedded rat heart was treated with DNase I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNase I treated sample (red). The cells were counterstained with DAPI (blue).This photo was taken by confocal microscope.

Paraffin embedded rat brain was treated with DNase I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNase I treated sample (red). The cells were counterstained with DAPI (blue).

Paraffin embedded mouse lung was treated with DNase I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNase I treated sample (red). The cells were counterstained with DAPI (blue).

Fluorescence microscope analysis of camptothecin-induced apoptosis of Hela cells.

Mixed samples of normal mouse spleen cells and DNase I treated mouse spleen cells were stained.

The effect of microRNA-663b in the inhibition of interleukin-1-induced nucleus pulposus cell apoptosis and inflammatory response.

IF: 2.200

Journal： JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY (2023)

DOI：10.26402/jpp.2023.10.09
Neural stem cell-derived exosomal FTO protects neuron from microglial inflammatory injury by inhibiting microglia NRF2 mRNA m6A modification

IF: 1.9

Journal： JOURNAL OF NEUROGENETICS (2023)

DOI：10.1080/01677063.2023.2259995
Dehydroevodiamine Targeting ACHE and ALDH3A1 Regulate Glycolytic Metabolism to Inhibit Hepatocellular Carcinoma Cell Apoptosis

IF: 1.8

Journal： Natural Product Communications (2023)

DOI：10.1177/1934578X231195153
AEBP1 exacerbates myocardial ischemia-reperfusion injury via inhibition of IκBα.

IF: 1.2

Journal： FOLIA MORPHOLOGICA (2023)

DOI：10.5603/fm.96874
Long-term monitoring of intravital biological processes using fluorescent protein-assisted NIR-II imaging

IF: 17.694

Journal： Nature Communications (2022)

DOI：10.1038/s41467-022-34274-w

PMID：36333308
Platelet membrane-camouflaged nanoparticles carry microRNA inhibitor against myocardial ischaemia?reperfusion injury

IF: 9.429

Journal： JOURNAL OF NANOBIOTECHNOLOGY (2022)

DOI：10.1186/s12951-022-01639-8

PMID：36195952
miR-126-3p containing exosomes derived from human umbilical cord mesenchymal stem cells promote angiogenesis and attenuate ovarian granulosa cell apoptosis in a preclinical rat model of premature ovarian failure

IF: 8.079

Journal： Stem Cell Research & Therapy (2022)

DOI：10.1186/s13287-022-03056-y

PMID：35883161
Monomethyl lithospermate alleviates ischemic stroke injury in middle cerebral artery occlusion mice in vivo and protects oxygen glucose deprivation/reoxygenation induced SHSY-5Y cells in vitro via activation of PI3K/Akt signaling.

IF: 5.988

Journal： Frontiers in Pharmacology (2022)

DOI：10.3389/fphar.2022.1024439

PMID：36313310

Reactivity：Rat

Sample Type：Tissue Slice
Hyperoxia Induced Bronchopulmonary Dysplasia-Like Inflammation via miR34a-TNIP2-IL-1β Pathway

IF: 3.569

Journal： Frontiers in Pediatrics (2022)

DOI：10.3389/fped.2022.805860

PMID：35433535

Reactivity：Human

Sample Type：sputum
Rosiglitazone attenuates hypoxia-induced renal cell apoptosis by inhibiting NF-κB signaling pathway in a PPARγ-dependent manner

IF: 3.222

Journal： RENAL FAILURE (2022)

DOI：10.1080/0886022X.2022.2148539

PMID：36420656

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