Phospho-SMAD3 (Ser425) Monoclonal Antibody
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- 宿主： Mouse
- 反应性： Human;Mouse;Rat
- 应用性： IHC-p
| Verified Samples |
Verified Samples in IHC: Human skin |
| Dilution | IHC 1:50-300 |
| Clonality | Monoclonal |
| Isotype | IgG |
| Immunogen | Synthetic Peptide of Phospho-Smad3 (Ser425) |
| Abbre | Phospho-SMAD3 (Ser425) |
| Synonyms | DKFZP586N0721, DKFZp686J10186, HSPC193, HST17436, JV15 2, JV15-2, JV152, LDS1C, LDS3, MAD, MAD (mothers against decapentaplegic Drosophila) homolog 3, MAD homolog 3, Mad homolog JV15 2, Mad protein homolog, hMAD 3, hMAD-3, hSMAD3, mothers against decapentaplegic homolog 3 |
| Swissprot | |
| Cellular Localization | Cytoplasm. Nucleus. Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4 (PubMed:15799969). Through the action of the phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1 (PubMed:16751101, PubMed:19289081). Co-localizes with LEMD3 at the nucleus inner membrane (PubMed:15601644). MAPK-mediated phosphorylation appears to have no effect on nuclear import (PubMed:19218245). PDPK1 prevents its nuclear translocation in response to TGF-beta (PubMed:17327236). |
| Concentration | 1 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH 7.4. |
| Purification Method | Protein A purification |
| Research Areas | Cancer, Epigenetics and Nuclear Signaling, Metabolism, Signal Transduction, Stem Cells |
| Clone No. | 4C5 |
| Conjugation | Unconjugated |
| Background | Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. |
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Sonodynamic Therapy Inhibits Fibrogenesis in Rat Cardiac Fibroblasts Induced by TGF-β1
IF: 4.652Journal： CELLULAR PHYSIOLOGY AND BIOCHEMISTRY (2016)PMID：27889772Reactivity：RatSample Type：Cell culture supernatant
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| E-AB-1024 | Goat Anti-Mouse IgG(H+L)(Biotin conjugated) | 500 μL , 120 μL , 60 μL , 30 μL , 1 mL |
| E-AB-1043 | Streptavidin(peroxidase/HRP conjugated) | 500 μL , 120 μL , 60 μL , 30 μL , 1 mL |
| E-AB-1196 | HRP-Goat Anti-Mouse IgG(H+L) preadsorbed | 500 μL , 120 μL , 1 mL |
| E-IR-R217 | 2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution) | 50 mL , 18 mL , 6 mL , 3 mL |
| E-IR-R220 | Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0) | 10mL , 3mL |
| E-IR-R221 | Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0) | 10mL , 3mL |
| E-IR-R304A | Western Blot Detection Kit | 50 Assays |
| E-IR-R304B | High Accuracy and Absorbability Western Blot Detection Kit | 50 Assays |
