RAW 264.7 Polarized M1 Macrophage Culture and Identification Kit
中文名称：M1型Raw264.7巨噬细胞极化培养和检测试剂盒
Price:
Size:
100 Assays
20 Assays
| 产品介绍 |
巨噬细胞作为具有异质性和多样性的免疫细胞，具有独特的形态和功能特性，参与炎症反应、自身免疫和损伤修复。巨噬细胞的极化在消灭病原体或维持组织稳态中起着至关重要的作用。近年来，人们普遍认为各种免疫相关疾病的发生发展受巨噬细胞极化的影响。在复杂多变的微环境下，巨噬细胞可以转化为不同的表型。一般来说，巨噬细胞至少有两种不同的极化：M1型巨噬细胞和M2型巨噬细胞。巨噬细胞在脂多糖(LPS)、干扰素-γ (IFN-γ)等物质的刺激下转化为M1型巨噬细胞。这类巨噬细胞可产生高水平的一氧化氮合酶(iNOS)、白细胞介素-6 (IL-6)、肿瘤坏死因子-a (TNF-a)，从而促进炎症反应。RAW 264.7(小鼠单核巨噬细胞白血病细胞)是破骨细胞、炎症研究最常用的体外模型之一，被广泛用于研究风湿性关节炎、骨质疏松症、骨质溶解、牙周炎等骨骼疾病。本试剂盒提供完整的RAW 264.7极化为M1型巨噬细胞的诱导分化和鉴定方案，为RAW 264.7极化为M1型巨噬细胞提供稳定可靠的支持。
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| 产品特点 |
本试剂盒提供完整的RAW 264.7极化为M1型巨噬细胞的诱导分化和鉴定方案，包括M1型巨噬细胞分化培养试剂、鉴定试剂，为RAW 264.7极化为M1型巨噬细胞提供稳定可靠的支持，提高了RAW 264.7极化为M1型巨噬细胞的培养和鉴定的效率及稳定性。
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| 产品应用 |
This kit provides a complete set of differentiation and identification protocols for RAW 264.7 polarized to M1 macrophages, including M1 macrophage differentiation differentiation and identification reagents, which provides stable and reliable support for the differentiation and identification of RAW 264.7 polarized to M1 macrophages, and improves the efficiency and stability of differentiation and identification of RAW 264.7 polarized to M1 macrophages.
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| 样本类型 |
RAW 264.7 细胞
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| 保存条件 |
该产品可在 2-8°C/-20°C避光保存1年有效，试剂盒组分的详细储存条件请参考说明书。
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| 保质期 |
12个月
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| 运输条件 |
冰袋
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RAW 264.7形态学观察：未处理的RAW 264.7(左)细胞呈现出圆形或椭圆形的形态；RAW 264.7分化的M1型巨噬细胞(右)呈现不规则形态，细胞质中含有颗粒状物质。
RAW 264.7的M1型巨噬细胞表型分析：RAW 264.7 细胞用RAW 264.7 Cell M1 Differentiation MIX(1000×)极化诱导48h(M1-48h)和未极化处理的空白RAW 264.7细胞(control-48h)培养48h，流式细胞仪检测分析。
RAW 264.7 细胞使用RAW 264.7 Cell M1 Differentiation MIX(1000×)极化诱导48h (M1-48h)和未极化处理的空白RAW 264.7细胞(control-48h)培养48h后收集上清,使用Mouse TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit(E-EL-M3063)/Mouse IL-12(Interleukin 12) ELISA Kit(E-EL-M3062)/Mouse IL-6(Interleukin 6) ELISA Kit(E-EL-M0044)/Mouse IL-1β(Interleukin 1 Beta) ELISA Kit(E-EL-M0037)检测并分析结果。
| E-AB-F09832D | PE Rat IgG2a, κ Isotype Control[2A3] | 100Tests , 20Tests , 100Tests×2 |
| E-AB-F09832E | APC Rat IgG2a, κ Isotype Control[2A3] | 100Tests , 20Tests , 100Tests×2 |
| E-AB-F09842C | FITC Rat IgG2b, κ Isotype Control[LTF-2] | 100Tests , 20Tests , 100Tests×2 |
| E-AB-F09842J | PerCP/Cyanine5.5 Rat IgG2b, κ Isotype Control[LTF-2] | 100Tests , 20Tests , 100Tests×2 |
| E-AB-F0994D | PE Anti-Mouse CD86 Antibody[GL-1] | 100Tests , 50Tests , 100Tests×2 |
| E-AB-F0995C | FITC Anti-Mouse F4/80 Antibody[CI:A3-1] | 100Tests , 50Tests , 100Tests×2 |
| E-AB-F0997A | Purified Anti-Mouse CD16/32 Antibody[2.4G2] | 100 μg , 25 μg |
| E-AB-F1081J | PerCP/Cyanine5.5 Anti-Mouse/Human CD11b Antibody[M1/70] | 100Tests , 50Tests , 100Tests×2 |
| E-AB-F1135E | APC Anti-Mouse CD206/MMR Antibody[C068C2] | 100Tests , 50Tests , 100Tests×2 |
| E-AB-F1207E | APC Anti-Mouse IL-6 Antibody[MP5-20F3] | 100Tests , 50Tests , 100Tests×2 |
| E-BC-K035-M | Nitric Oxide (NO) Colorimetric Assay Kit | 96 T (80 samples) , 48 T (32 samples) |
| E-EL-M0037 | Mouse IL-1β(Interleukin 1 Beta) ELISA Kit | 96 T , 48 T , 96 T × 5 |
| E-EL-M0044 | Mouse IL-6(Interleukin 6) ELISA Kit | 96 T , 48 T , 96 T × 5 |
| E-EL-M3062 | Mouse IL-12(Interleukin 12) ELISA Kit | 96 T , 48 T , 96 T × 5 |
| E-EL-M3063 | Mouse TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit | 96 T , 48 T , 96 T × 5 |
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