S-tag Monoclonal Antibody
Price:
Size:
100 μL
25 μL
- 宿主： Mouse
- 反应性： All
- 应用性： WB
| Verified Samples |
Verified Samples in WB: S-Tag fusion protein (40kDa) |
| Dilution | WB 1:2000-4000 |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Immunogen | Synthetic peptide corresponding to S tag conjugated to keyhole limpet haemocyanin. |
| Abbre | S-tag |
| Synonyms | KETAAAKFERQHMDS tag, S peptide, S peptide epitope tag |
| Calculated MW | 40 kDa |
| Observed MW | 40 kDa |
| Concentration | 1 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Protein A/G Purification |
| Clone No. | 2A4 |
| Conjugation | Unconjugated |
| Background | S tag is an epitope tag composed of a 15 residue peptide, KETAAAKFERQHMDS, derived from the pancreatic ribonuclease A. This interactive complex (S‐tag/S‐protein) results in micromolar affinity – dependent upon pH, temperature, and ionic strength . The S‐tag itself enables colorimetric detection via western blot analysis or a rapidly detected assay dependent upon ribonucleolytic reconstitution and activity. |
Western blotting with anti-S-Tag monoclonal antibody at dilution of 1:1000.Lane 1: S tag transfected HEK 293 cell lysates, lane 2: HEK 293 whole cell lysate
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