Strep-Tag II Monoclonal Antibody
Price:
Size:
100 μL
25 μL
- 宿主： Mouse
- 反应性： All
- 应用性： WB
| Verified Samples |
Verified Samples in WB: Strep Ⅱ-Tag fused recombinant protein |
| Dilution | WB 1:1000-2000 |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Immunogen | Synthetic peptide corresponding to Strep II tag conjugated to keyhole limpet haemocyanin. |
| Abbre | Strep-Tag II |
| Synonyms | Strep II, Strep Tag II |
| Calculated MW | 45 kDa |
| Observed MW | 45 kDa |
| Concentration | 1 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Protein A/G Purification |
| Clone No. | 4H10 |
| Conjugation | Unconjugated |
| Background | The Strep-tag II is a synthetic peptide consisting of eight amino acids ( WSHPQFEK ). This peptide sequence exhibits intrinsic affinity towards Strep-Tactin, a specifically engineered streptavidin and can be N- or C- terminally fused to recombinant protein. The Strep-tag II peptide was developed as an affinity tool for the purification of corresponding fusion proteins on streptavidin columns. The peptide recognizes the same pocket of streptavidin where the natural ligand is normally bound so that biotin or its chemical derivatives can be used for competitive elution. |
Western Blotting of Strep Ⅱ-Tag fused recombinant protein with Anti-Strep Ⅱ mouse monoclonal antibody at dilution of 1:1000.
| E-AB-1001 | Goat Anti-Mouse IgG(H+L)(peroxidase/HRP conjugated) | 500 μL , 120 μL , 60 μL , 30 μL , 1 mL |
| E-AB-1024 | Goat Anti-Mouse IgG(H+L)(Biotin conjugated) | 500 μL , 120 μL , 60 μL , 30 μL , 1 mL |
| E-AB-1043 | Streptavidin(peroxidase/HRP conjugated) | 500 μL , 120 μL , 60 μL , 30 μL , 1 mL |
| E-AB-1196 | HRP-Goat Anti-Mouse IgG(H+L) preadsorbed | 500 μL , 120 μL , 1 mL |
| E-IR-R304A | Western Blot Detection Kit | 50 Assays |
| E-IR-R304B | High Accuracy and Absorbability Western Blot Detection Kit | 50 Assays |
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