TNF alpha Monoclonal Antibody-Elabscience伊莱瑞特【官网】

TNF alpha Monoclonal Antibody

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货号：E-AB-22116
文献（7）
说明书

说明书

Price: ¥2310.00 ¥1420.00 ¥580.00

Size:

120 μL 60 μL 20 μL

宿主： Mouse
反应性： Human;Mouse;Rat
应用性： WB;IHC-p

Verified Samples	Verified Samples in WB: Recombinant protein Verified Samples in IHC: Human tonsil
Dilution	WB 1:1000-1:3000, IHC 1:50-1:200
Clonality	Monoclonal
Isotype	IgG
Immunogen	Synthetic Peptide
Abbre	TNF α
Synonyms	APC1, APC1 protein, Cachectin, DIF, Differentiation inducing factor, Macrophage cytotoxic factor, TNF, TNF superfamily, TNF-a, TNF-alpha, TNFA, TNFSF2, Tnf, Tumor n, Tumor necrosis factor, Tumor necrosis factor alpha, macrophage derived, member 2, monocyte derived
Swissprot	P01375
Observed MW	17 ,25kDa
Cellular Localization	Secreted and Cell membrane.
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH 7.4.
Purification Method	Protein A purification
Research Areas	Cancer, Cardiovascular, Metabolism, Immunology, Signal Transduction
Clone No.	3D8
Conjugation	Unconjugated
Background	TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

Western Blot analysis of Recombinant human TNF α protein using TNF alpha Monoclonal Antibody at dilution of 1:2000.

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue using TNF alpha Monoclonal Antibody at dilution of 1:50.

Therapeutic Potential of the Linalool against Cadmium-Induced Testicular Tissue Damage

IF: 3.600

Journal： JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY (2024)

DOI：10.1016/j.jtemb.2024.127455

Reactivity：Rat

Sample Type：testicular tissue
Kaempferol sophoroside glucoside mitigates acetaminophen-induced hepatotoxicity: Role of Nrf2/NF-κB and JNK/ASK-1 signaling pathways

IF: 3.400

Journal： Heliyon (2024)

DOI：10.1016/j.heliyon.2024.e31448

Reactivity：Mouse

Sample Type：liver
The Effect of Thymoquinone on the TNF-α/OTULIN/NF-κB Axis Against Cisplatin-?nduced Testicular Tissue Damage

IF: 2.600

Journal： Reproductive Sciences (2024)

DOI：10.1007/s43032-024-01567-y

Reactivity：Rat

Sample Type：testicular tissue
Resveratrol may dose-dependently modulate nephrin and OTULIN levels in a doxorubicin-induced nephrotoxicity model

IF: 3.200

Journal： TOXICOLOGY MECHANISMS AND METHODS (2023)

DOI：10.1080/15376516.2023.2268717

PMID：37807854

Reactivity：Rat

Sample Type：kidney
Microbiota dysbiosis influences immune system and muscle pathophysiology of dystrophin-deficient mice

IF: 14.260

Journal： EMBO Molecular Medicine (2022)

DOI：10.15252/emmm.202216244

Reactivity：Mouse

Sample Type：Tibialis anterior skeletal muscles ,colonic tissues
Synergistic neuroprotective effects of hyperbaric oxygen and methylprednisolone following contusive spinal cord injury in rat

IF: 1.985

Journal： JOURNAL OF SPINAL CORD MEDICINE (2021)

DOI：10.1080/10790268.2021.1896275

Reactivity：Rat

Sample Type：spinal cord tissue
FCN-A mediates the inflammatory response and the macrophage polarization in Aspergillus fumigatus keratitis of mice by activating the MAPK signaling pathway

IF: 3.943

Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2020)

DOI：10.1016/j.intimp.2020.106473

PMID：32272397

Reactivity：Mouse

Sample Type：Cell lysate,Tissue homogenate

E-AB-1001	Goat Anti-Mouse IgG(H+L)(peroxidase/HRP conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1024	Goat Anti-Mouse IgG(H+L)(Biotin conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1043	Streptavidin(peroxidase/HRP conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1196	HRP-Goat Anti-Mouse IgG(H+L) preadsorbed	500 μL , 120 μL , 1 mL
E-IR-R217	2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution)	50 mL , 18 mL , 6 mL , 3 mL
E-IR-R220	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0)	10mL , 3mL
E-IR-R221	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0)	10mL , 3mL
E-IR-R304A	Western Blot Detection Kit	50 Assays
E-IR-R304B	High Accuracy and Absorbability Western Blot Detection Kit	50 Assays