TNF alpha Monoclonal Antibody-Elabscience伊莱瑞特【官网】

TNF alpha Monoclonal Antibody

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货号：E-AB-22159
文献（17）
说明书

说明书

Price: ¥2310.00 ¥1420.00 ¥580.00

Size:

120 μL 60 μL 20 μL

宿主： Mouse
反应性： Human;Mouse;Rat
应用性： WB;IHC-p

Verified Samples	Verified Samples in WB: Recombinant protein Verified Samples in IHC: Human tonsil
Dilution	WB 1:1000-1:3000, IHC 1:50-1:200
Clonality	Monoclonal
Isotype	IgG
Immunogen	Synthetic Peptide
Abbre	TNF α
Synonyms	APC1, APC1 protein, Cachectin, DIF, Differentiation inducing factor, Macrophage cytotoxic factor, TNF, TNF superfamily, TNF-a, TNF-alpha, TNFA, TNFSF2, Tnf, Tumor n, Tumor necrosis factor, Tumor necrosis factor alpha, macrophage derived, member 2, monocyte derived
Swissprot	P01375
Observed MW	17 ,25kDa
Cellular Localization	Secreted and Cell membrane.
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH 7.4.
Purification Method	Protein A purification
Research Areas	Cancer, Cardiovascular, Metabolism, Immunology, Signal Transduction
Clone No.	Q34
Conjugation	Unconjugated
Background	TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

Western Blot analysis of Recombinant Human TNF a Protein using TNF alpha Monoclonal Antibody at dilution of 1:2000.

Immunohistochemistry of paraffin-embedded Human tonsil tissue using TNF alpha Monoclonal Antibody at dilution of 1:50.

Implication of Let7b/AhR/ARNT/HMGB1/RAGE cascade in neuroplasticity disturbances induced by glucocorticoids and the promising reversible effect of 3,3 diindolymethane: Bidirectional crosstalk of Aryl hydrocarbon receptors

IF: 5.300

Journal： BIOCHEMICAL PHARMACOLOGY (2024)

DOI：10.1016/j.bcp.2024.116692

Reactivity：Rat

Sample Type：cerebral cortex tissue
Targeting SRD5A1 and MMP-2/NLRP3/TGF-β1 axis alleviates the amlodipine-induced gingival hyperplasia in rats: Emerging role of saw palmetto and folic acid

IF: 3.900

Journal： FOOD AND CHEMICAL TOXICOLOGY (2024)

DOI：10.1016/j.fct.2024.114731

Reactivity：Rat

Sample Type：gingival tissue
Therapeutic Potential of the Linalool against Cadmium-Induced Testicular Tissue Damage

IF: 3.600

Journal： JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY (2024)

DOI：10.1016/j.jtemb.2024.127455

Reactivity：Rat

Sample Type：testicular tissue
Kaempferol sophoroside glucoside mitigates acetaminophen-induced hepatotoxicity: Role of Nrf2/NF-κB and JNK/ASK-1 signaling pathways

IF: 3.400

Journal： Heliyon (2024)

DOI：10.1016/j.heliyon.2024.e31448

Reactivity：Mouse

Sample Type：liver
The Effect of Thymoquinone on the TNF-α/OTULIN/NF-κB Axis Against Cisplatin-?nduced Testicular Tissue Damage

IF: 2.600

Journal： Reproductive Sciences (2024)

DOI：10.1007/s43032-024-01567-y

Reactivity：Rat

Sample Type：testicular tissue
Traumatic brain injury-induced peripheral damage: The dynamics of the inflammatory and autophagy pathway from acute to chronic stages

IF: 1.500

Journal： FOLIA NEUROPATHOLOGICA (2024)

DOI：10.5114/fn.2024.140788

Reactivity：Mouse

Sample Type：kidney tissue,liver tissue
Amisulpride attenuates 5-fluorouracil-induced cognitive deficits via modulating hippocampal Wnt/GSK-3β/β-catenin signaling in Wistar rats

IF: 5.600

Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2023)

DOI：10.1016/j.intimp.2023.110945

PMID：37716161

Reactivity：Rat

Sample Type：hippocampal tissue
Protective role of olive oil extract of propolis on short and long-term administration of tamoxifen in rats

IF: 4.500

Journal： ENVIRONMENTAL TOXICOLOGY (2023)

DOI：10.1002/tox.24091

PMID：38108573

Reactivity：Rat

Sample Type：kidney tissue,liver tissue,ovary tissue
Evaluation of the protective role of resveratrol against sepsis caused by LPS via TLR4/NF-κB/TNF-α signaling pathways: Experimental study

IF: 3.600

Journal： CELL BIOCHEMISTRY AND FUNCTION (2023)

DOI：10.1002/cbf.3790

PMID：36970761

Reactivity：Rat

Sample Type：kidney,liver
Immunomodulatory effects of chronic trichinellosis on Toxoplasma gondii RH virulent strain in experimental rats

IF: 3.400

Journal： Pathogens and Global Health (2023)

DOI：10.1080/20477724.2023.2191233

PMID：36922743PMC10177679

Reactivity：Rat

Sample Type：brain tissues

E-AB-1001	Goat Anti-Mouse IgG(H+L)(peroxidase/HRP conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1024	Goat Anti-Mouse IgG(H+L)(Biotin conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1043	Streptavidin(peroxidase/HRP conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1196	HRP-Goat Anti-Mouse IgG(H+L) preadsorbed	500 μL , 120 μL , 1 mL
E-IR-R217	2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution)	50 mL , 18 mL , 6 mL , 3 mL
E-IR-R220	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0)	10mL , 3mL
E-IR-R221	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0)	10mL , 3mL
E-IR-R304A	Western Blot Detection Kit	50 Assays
E-IR-R304B	High Accuracy and Absorbability Western Blot Detection Kit	50 Assays