Total Superoxide Dismutase (T-SOD) Activity Assay Kit (Hydroxylamine Method)
中文名称:总超氧化物歧化酶(T-SOD)比色法测试盒(羟胺法)

Price:
- 样本类型: 血清(浆);尿液;动植物组织;细胞;细胞上清
- 检测仪器及波长: 酶标仪(540-560 nm,最佳检测波长为550 nm)
- 研究领域: 氧化应激;植物生理系列
生化试剂盒数量计算器x
需测样本数:
单个样本测量孔数:
试剂盒测样本孔数:
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所需盒子量:
检测原理 |
通过黄嘌呤及黄嘌呤氧化酶反应系统产生超氧阴离子自由基(O2-),它氧化羟胺形成亚硝酸盐,在显色剂作用下产生紫红色化合物。当反应体系中加入SOD后,催化超氧阴离子自由基发生歧化反应,使形成的亚硝酸盐减少,进而产生的紫红色变浅。根据样品管与对照管中的吸光度差值,来计算SOD的活性。
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指标简称 | T-SOD |
灵敏度 | 2.4 U/mL |
检测范围 | 2.4-61 U/mL |
精密度 | 批间差:5.6% | 批内差:5.5% |
检测时间 | 1 h 10 min |
检测仪器及波长 | 酶标仪(540-560 nm,最佳检测波长为550 nm) |
保存条件 | 该产品可在 2-8°C/-20°C避光条件下保存6个月,具体试剂组分的保存条件请参考说明书。 |
有效期 | 6个月 |
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Q1:检测细胞样本SOD,用RIPA裂解液处理细胞行不行?
RIPA裂解液中SDS对酶活测定会有干扰,因此不建议使用RIPA裂解液进行组织或细胞的处理。SOD试剂盒要求样本中不能含有SDS、Tween20、NP-40、Triton X-100等去污剂,不能含有DTT、2-巯基乙醇等还原性试剂。
Q2:检测SOD,血液样本用EDTA抗凝行不行?
SOD的酶活中心为金属离子,用EDTA做抗凝剂会螯合金属离子,可能会造成SOD酶活降低,影响测值结果。