V5-Tag Monoclonal Antibody
Price:
Size:
100 μL
25 μL
- 宿主： Mouse
- 反应性： All
- 应用性： WB
| Verified Samples |
Verified Samples in WB: V5-Tag fused recombinant protein (43kDa) |
| Dilution | WB 1:1000-2000, IP 3ug/sample, IF 1:100-200 |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Immunogen | Synthetic peptide corresponding to V5 tag conjugated to keyhole limpet haemocyanin. |
| Abbre | V5-Tag |
| Synonyms | GKPIPNPLLGLDST tag, Protein Rev, V5 epitope tag, rev, GKPIPNPLLGLDST epitope tag, Regulator of expression of viral proteins |
| Calculated MW | 40 kDa |
| Observed MW | 40 kDa |
| Concentration | 1 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Protein A/G Purification |
| Clone No. | 8E10 |
| Conjugation | Unconjugated |
| Background | The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD) |
Western blotting with anti-V5-Tag monoclonal antibody at dilution of 1:1000.Lane 1: V5 tag transfected HEK 293 cell lysates, lane 2: HEK 293 whole cell lysate
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| E-AB-1043 | Streptavidin(peroxidase/HRP conjugated) | 500 μL , 120 μL , 60 μL , 30 μL , 1 mL |
| E-AB-1196 | HRP-Goat Anti-Mouse IgG(H+L) preadsorbed | 500 μL , 120 μL , 1 mL |
| E-IR-R304A | Western Blot Detection Kit | 50 Assays |
| E-IR-R304B | High Accuracy and Absorbability Western Blot Detection Kit | 50 Assays |
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