alpha Tubulin Monoclonal Antibody

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- 宿主: Mouse
- 反应性: Human;Mouse;Rat
- 应用性: WB;IHC-p;IF;IP
Verified Samples |
Verified Samples in WB: Hela, Rat brain, Mouse brain Verified Samples in IHC: Mouse liver Verified Samples in IF: Mouse liver |
Dilution | WB 1:500-10000, IHC 1:50-300, IF 1:50-1:200, IP 1:100-1:300 |
Clonality | Monoclonal |
Isotype | IgG |
Immunogen | Recombinant Protein |
Abbre | α-tubulin |
Synonyms | Alpha-tubulin 3, Alpha-tubulin ubiquitous, TUBA1A, TUBA1B, TUBA3, Tubulin B-alpha-1, Tubulin K-alpha-1, Tubulin alpha-1A chain, Tubulin alpha-1B chain, Tubulin alpha-3 chain, Tubulin alpha-ubiquitous chain |
Swissprot | |
Calculated MW | 50 kDa |
Observed MW | 52 kDa |
Cellular Localization | Cytoskeleton, cytoplasmic microtubule, microtubule, microtubule cytoskeleton, Cytosol, Endosome, recycling endosome, Extracellular region or secreted, extracellular exosome, Nucleus, Other locations: cytoplasmic ribonucleoprotein granule, membrane raft, myelin sheath. |
Concentration | 1 mg/mL |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Buffer | PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH 7.4. |
Purification Method | Protein A purification |
Research Areas | Cancer, Signal Transduction |
Clone No. | 5M5 |
Conjugation | Unconjugated |
Background | There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs. |
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