alpha Tubulin Monoclonal Antibody（E-AB-20036）|-Elabscience伊莱瑞特【官网】

alpha Tubulin Monoclonal Antibody

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货号：E-AB-20036
文献（39）
说明书

说明书

Price: ¥2835.00 ¥875.00 ¥548.00 ¥295.00 ¥1975.00

Size:

500 μL 120 μL 60 μL 30 μL 5 × 60 μL

宿主： Mouse
反应性： Human;Mouse;Rat
应用性： WB;IHC-p;IF;IP

Verified Samples	Verified Samples in WB: Hela, Rat brain, Mouse brain Verified Samples in IHC: Mouse liver Verified Samples in IF: Mouse liver
Dilution	WB 1:500-10000, IHC 1:50-300, IF 1:50-1:200, IP 1:100-1:300
Clonality	Monoclonal
Isotype	IgG
Immunogen	Recombinant Protein
Abbre	α-tubulin
Synonyms	Alpha-tubulin 3, Alpha-tubulin ubiquitous, TUBA1A, TUBA1B, TUBA3, Tubulin B-alpha-1, Tubulin K-alpha-1, Tubulin alpha-1A chain, Tubulin alpha-1B chain, Tubulin alpha-3 chain, Tubulin alpha-ubiquitous chain
Swissprot	Q71U36, P68363
Calculated MW	50 kDa
Observed MW	52 kDa
Cellular Localization	Cytoskeleton, cytoplasmic microtubule, microtubule, microtubule cytoskeleton, Cytosol, Endosome, recycling endosome, Extracellular region or secreted, extracellular exosome, Nucleus, Other locations: cytoplasmic ribonucleoprotein granule, membrane raft, myelin sheath.
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH 7.4.
Purification Method	Protein A purification
Research Areas	Cancer, Signal Transduction
Clone No.	5M5
Conjugation	Unconjugated
Background	There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs.

Western Blot analysis of 1) Hela, 2) Rat brian, 3) Mouse brain using alpha Tubulin Monoclonal Antibody at dilution of 1:5000.

Immunohistochemistry of paraffin-embedded mouse liver using alpha Tubulin Monoclonal Antibody at dilution of 1:200

Immunofluorescence analysis of Mouse liver tissue using alpha Tubulin Monoclonal Antibody at dilution of 1:200.

MiR-27b attenuates mitochondrial oxidative stress and inflammation in endothelial cells

IF: 11.400

Journal： Redox Biology (2023)

DOI：10.1016/j.redox.2023.102681

PMID：37003179

Reactivity：Human
ANXA1 promotes tumor immune evasion by binding PARP1 and upregulating Stat3-induced expression of PD-L1 in multiple cancers

IF: 10.1

Journal： Cancer Immunology Research (2023)

DOI：10.1158/2326-6066.CIR-22-0896

PMID：37566399

Reactivity：Human
MiR-15b-5p and PCSK9 inhibition reduces lipopolysaccharide-induced endothelial dysfunction by targeting SIRT4

IF: 8.3

Journal： CELLULAR & MOLECULAR BIOLOGY LETTERS (2023)

DOI：10.1186/s11658-023-00482-5

PMID：37587410

Reactivity：Human
SGLT2 breast expression could affect the cardiovascular performance in pre-menopausal women with fatty vs. non fatty breast via over-inflammation and sirtuins’ down regulation

IF: 8.000

Journal： European Journal of Internal Medicine (2023)

DOI：10.1016/j.ejim.2023.04.012

PMID：37062642

Reactivity：Human

Sample Type：breast tissue
Whey Improves In Vitro Endothelial Mitochondrial Function and Metabolic Redox Status in Diabetic State

IF: 7.0

Journal： Antioxidants (2023)

DOI：10.3390/antiox12061311

PMID：37372041

Reactivity：Human
Filamin A cooperates with the androgen receptor in preventing skeletal muscle senescence

IF: 7.0

Journal： Cell Death Discovery (2023)

DOI：10.1038/s41420-023-01737-y

PMID：38040692

Reactivity：Mouse
Evidence of an anti-inflammatory effect of PCSK9 inhibitors within the human atherosclerotic plaque

IF: 5.3

Journal： ATHEROSCLEROSIS (2023)

DOI：10.1016/j.atherosclerosis.2023.06.971

PMID：37422356

Reactivity：Human

Sample Type：plaques
MiR-148a-3p Promotes Colorectal Cancer Cell Ferroptosis by Targeting SLC7A11

IF: 5.2

Journal： Cancers (2023)

DOI：10.3390/cancers15174342

PMID：37686618

Reactivity：Human
The interplay between kisspeptin and endocannabinoid systems modulates male hypothalamic and gonadic control of reproduction in vivo

IF: 5.2

Journal： Frontiers in Endocrinology (2023)

DOI：10.3389/fendo.2023.1269334

PMID：37900144

Reactivity：Rat

Sample Type：testis
Expression of RSPH6A in the first wave of rat spermatogenesis and oxidative stress conditions: Attenuation by melatonin

IF: 3.4

Journal： Reproductive Medicine and Biology (2023)

DOI：10.1002/rmb2.12542

PMID：37795044

Reactivity：Rat

Sample Type：testis,spermatozoa (SPZ)

E-AB-1001	Goat Anti-Mouse IgG(H+L)(peroxidase/HRP conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1043	Streptavidin(peroxidase/HRP conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1024	Goat Anti-Mouse IgG(H+L)(Biotin conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1011	Goat Anti-Mouse IgG(H+L)(Cyanine3 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1015	Goat Anti-Mouse IgG(H+L)(FITC conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1059	Goat Anti-Mouse IgG(H+L)(Elab Fluor® 594 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-IR-R304A	Western Blot Detection Kit	50 Assays
E-IR-R304B	High Accuracy and Absorbability Western Blot Detection Kit	50 Assays
E-IR-R217	2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution)	50 mL , 18 mL , 6 mL , 3 mL
E-IR-R220	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0)	10 mL , 3 mL
E-IR-R221	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0)	10 mL , 3 mL
E-AB-1076	Goat Anti-Mouse IgG(H+L)(Elab Fluor® 647 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1196	HRP-Goat Anti-Mouse IgG(H+L) preadsorbed	500 μL , 120 μL , 1 mL