alpha Tubulin Monoclonal Antibody（E-AB-20036）|-Elabscience伊莱瑞特【官网】

alpha Tubulin Monoclonal Antibody

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货号：E-AB-20036
文献（39）
说明书

说明书

Price: ¥2835.00 ¥875.00 ¥548.00 ¥295.00 ¥1975.00

Size:

500 μL 120 μL 60 μL 30 μL 5 × 60 μL

宿主： Mouse
反应性： Human;Mouse;Rat
应用性： WB;IHC-p;IF;IP

Verified Samples	Verified Samples in WB: Hela, Rat brain, Mouse brain Verified Samples in IHC: Mouse liver Verified Samples in IF: Mouse liver
Dilution	WB 1:500-10000, IHC 1:50-300, IF 1:50-1:200, IP 1:100-1:300
Clonality	Monoclonal
Isotype	IgG
Immunogen	Recombinant Protein
Abbre	α-tubulin
Synonyms	Alpha-tubulin 3, Alpha-tubulin ubiquitous, TUBA1A, TUBA1B, TUBA3, Tubulin B-alpha-1, Tubulin K-alpha-1, Tubulin alpha-1A chain, Tubulin alpha-1B chain, Tubulin alpha-3 chain, Tubulin alpha-ubiquitous chain
Swissprot	Q71U36, P68363
Calculated MW	50 kDa
Observed MW	52 kDa
Cellular Localization	Cytoskeleton, cytoplasmic microtubule, microtubule, microtubule cytoskeleton, Cytosol, Endosome, recycling endosome, Extracellular region or secreted, extracellular exosome, Nucleus, Other locations: cytoplasmic ribonucleoprotein granule, membrane raft, myelin sheath.
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH 7.4.
Purification Method	Protein A purification
Research Areas	Cancer, Signal Transduction
Clone No.	5M5
Conjugation	Unconjugated
Background	There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs.

Western Blot analysis of 1) Hela, 2) Rat brian, 3) Mouse brain using alpha Tubulin Monoclonal Antibody at dilution of 1:5000.

Immunohistochemistry of paraffin-embedded mouse liver using alpha Tubulin Monoclonal Antibody at dilution of 1:200

Immunofluorescence analysis of Mouse liver tissue using alpha Tubulin Monoclonal Antibody at dilution of 1:200.

Morphological and molecular changes in the Harderian gland of streptozotocin-induced diabetic rats

IF: 2.8

Journal： Journal of Experimental Zoology Part A-Ecological and Integrative Physiology (2023)

DOI：10.1002/jez.2741

PMID：37522474

Reactivity：Rat

Sample Type：Harderian gland (HG)
Autophagic event and metabolomic disorders unveil cellular toxicity of environmental microplastics on marine polychaete Hediste diversicolor

IF: 9.988

Journal： ENVIRONMENTAL POLLUTION (2022)

DOI：10.1016/j.envpol.2022.119106

PMID：35248622

Reactivity：Hediste diversicolor

Sample Type：pidermis, epithelial,connective tissue
SIRT3 Modulates Endothelial Mitochondrial Redox State during Insulin Resistance

IF: 7.675

Journal： Antioxidants (2022)

DOI：10.3390/antiox11081611

PMID：36009329

Reactivity：Human
Milk Exosomal miR-27b Worsen Endoplasmic Reticulum Stress Mediated Colorectal Cancer Cell Death

IF: 6.706

Journal： Nutrients (2022)

DOI：10.3390/nu14235081

PMID：36501111

Reactivity：Human
New Insight on the In Vitro Effects of Melatonin in Preserving Human Sperm Quality

IF: 6.208

Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022)

DOI：10.3390/ijms23095128

PMID：35563519

Reactivity：Human

Sample Type：Spermatozoa (SPZ)
Buffalo Milk Whey Activates Necroptosis and Apoptosis in a Xenograft Model of Colorectal Cancer

IF: 6.208

Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022)

DOI：10.3390/ijms23158464

PMID：35955595

Reactivity：Mouse

Sample Type：HCT116 cell-xenograft
Breed and Feeding System Impact the Bioactive Anti-Inflammatory Properties of Bovine Milk

IF: 6.208

Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022)

DOI：10.3390/ijms231911088

PMID：36232386

Reactivity：Human
Cytotoxic Potential of the Marine Diatom Thalassiosira rotula: Insights into Bioactivity of 24-Methylene Cholesterol

IF: 6.085

Journal： Marine Drugs (2022)

DOI：10.3390/md20100595

PMID：36286419

Reactivity：Human
Differential Expression of Kisspeptin System and Kisspeptin Receptor Trafficking during Spermatozoa Transit in the Epididymis

IF: 4.141

Journal： Genes (2022)

DOI：10.3390/genes13020295

PMID：35205340

Reactivity：Rat

Sample Type：epididymis
Diet-derived ergothioneine induces necroptosis in colorectal cancer cells by activating the SIRT3/MLKL pathway

IF: 3.864

Journal： FEBS LETTERS (2022)

DOI：10.1002/1873-3468.14310

PMID：35122251

Reactivity：Human

E-AB-1001	Goat Anti-Mouse IgG(H+L)(peroxidase/HRP conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1043	Streptavidin(peroxidase/HRP conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1024	Goat Anti-Mouse IgG(H+L)(Biotin conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1011	Goat Anti-Mouse IgG(H+L)(Cyanine3 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1015	Goat Anti-Mouse IgG(H+L)(FITC conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1059	Goat Anti-Mouse IgG(H+L)(Elab Fluor® 594 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-IR-R304A	Western Blot Detection Kit	50 Assays
E-IR-R304B	High Accuracy and Absorbability Western Blot Detection Kit	50 Assays
E-IR-R217	2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution)	50 mL , 18 mL , 6 mL , 3 mL
E-IR-R220	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0)	10 mL , 3 mL
E-IR-R221	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0)	10 mL , 3 mL
E-AB-1076	Goat Anti-Mouse IgG(H+L)(Elab Fluor® 647 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1196	HRP-Goat Anti-Mouse IgG(H+L) preadsorbed	500 μL , 120 μL , 1 mL