alpha Tubulin Monoclonal Antibody（E-AB-20036）|-Elabscience伊莱瑞特【官网】

alpha Tubulin Monoclonal Antibody

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货号：E-AB-20036
文献（39）
说明书

说明书

Price: ¥2835.00 ¥875.00 ¥548.00 ¥295.00 ¥1975.00

Size:

500 μL 120 μL 60 μL 30 μL 5 × 60 μL

宿主： Mouse
反应性： Human;Mouse;Rat
应用性： WB;IHC-p;IF;IP

Verified Samples	Verified Samples in WB: Hela, Rat brain, Mouse brain Verified Samples in IHC: Mouse liver Verified Samples in IF: Mouse liver
Dilution	WB 1:500-10000, IHC 1:50-300, IF 1:50-1:200, IP 1:100-1:300
Clonality	Monoclonal
Isotype	IgG
Immunogen	Recombinant Protein
Abbre	α-tubulin
Synonyms	Alpha-tubulin 3, Alpha-tubulin ubiquitous, TUBA1A, TUBA1B, TUBA3, Tubulin B-alpha-1, Tubulin K-alpha-1, Tubulin alpha-1A chain, Tubulin alpha-1B chain, Tubulin alpha-3 chain, Tubulin alpha-ubiquitous chain
Swissprot	Q71U36, P68363
Calculated MW	50 kDa
Observed MW	52 kDa
Cellular Localization	Cytoskeleton, cytoplasmic microtubule, microtubule, microtubule cytoskeleton, Cytosol, Endosome, recycling endosome, Extracellular region or secreted, extracellular exosome, Nucleus, Other locations: cytoplasmic ribonucleoprotein granule, membrane raft, myelin sheath.
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH 7.4.
Purification Method	Protein A purification
Research Areas	Cancer, Signal Transduction
Clone No.	5M5
Conjugation	Unconjugated
Background	There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs.

Western Blot analysis of 1) Hela, 2) Rat brian, 3) Mouse brain using alpha Tubulin Monoclonal Antibody at dilution of 1:5000.

Immunohistochemistry of paraffin-embedded mouse liver using alpha Tubulin Monoclonal Antibody at dilution of 1:200

Immunofluorescence analysis of Mouse liver tissue using alpha Tubulin Monoclonal Antibody at dilution of 1:200.

Potential protective effect of lactic acid bacteria against zearalenone causing reprotoxicity in male mice

IF: 3.035

Journal： TOXICON (2022)

DOI：10.1016/j.toxicon.2022.02.011

PMID：35181403

Reactivity：Mouse

Sample Type：testis
Integrative Transcriptomic, Proteomic and Functional Analysis Reveals ATP1B3 as a Diagnostic and Potential Therapeutic Target in Hepatocellular Carcinoma

IF: 7.561

Journal： Frontiers in Immunology (2021)

DOI：10.3389/fimmu.2021.636614

PMID：33868261

Reactivity：Human
Sodium-glucose co-transporter2 expression and inflammatory activity in diabetic atherosclerotic plaques: Effects of sodium-glucose co-transporter2 inhibitor treatment

IF: 7.422

Journal： Molecular Metabolism (2021)

DOI：10.1016/j.molmet.2021.101337

PMID：34500107

Reactivity：Human
SIRT3 and Metabolic Reprogramming Mediate the Antiproliferative Effects of Whey in Human Colon Cancer Cells

IF: 6.639

Journal： Cancers (2021)

DOI：10.3390/cancers13205196

PMID：34680344

Reactivity：Human
Preliminary Investigation on the Involvement of Cytoskeleton-Related Proteins, DAAM1 and PREP, in Human Testicular Disorders

IF: 5.924

Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021)

DOI：10.3390/ijms22158094

PMID：34360857

Reactivity：Human

Sample Type：testicular
Colorectal Cancer Apoptosis Induced by Dietary δ-Valerobetaine Involves PINK1/Parkin Dependent-Mitophagy and SIRT3

IF: 5.924

Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021)

DOI：10.3390/ijms22158117

PMID：34360883

Reactivity：Human
Kisspeptin Receptor on the Sperm Surface Reflects Epididymal Maturation in the Dog

IF: 5.924

Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021)

DOI：10.3390/ijms221810120

PMID：34576283

Reactivity：Dog

Sample Type：testis
Preliminary Investigation on the Ameliorative Role Exerted by D-Aspartic Acid in Counteracting Ethane Dimethane Sulfonate (EDS) Toxicity in the Rat Testis

IF: 2.752

Journal： Animals (2021)

DOI：10.3390/ani11010133

PMID：33435542

Reactivity：Rat

Sample Type：Tissue
ANXA1?derived peptides suppress gastric and colon cancer cell growth by targeting EphA2 degradation Corrigendum in /10.3892/ijo.2020.5149

IF: 3.899

Journal： INTERNATIONAL JOURNAL OF ONCOLOGY (2020)

DOI：10.3892/ijo.2020.5119

PMID：32901832

Reactivity：Human

Sample Type：Cell culture supernatant

E-AB-1001	Goat Anti-Mouse IgG(H+L)(peroxidase/HRP conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1043	Streptavidin(peroxidase/HRP conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1024	Goat Anti-Mouse IgG(H+L)(Biotin conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1011	Goat Anti-Mouse IgG(H+L)(Cyanine3 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1015	Goat Anti-Mouse IgG(H+L)(FITC conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1059	Goat Anti-Mouse IgG(H+L)(Elab Fluor® 594 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-IR-R304A	Western Blot Detection Kit	50 Assays
E-IR-R304B	High Accuracy and Absorbability Western Blot Detection Kit	50 Assays
E-IR-R217	2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution)	50 mL , 18 mL , 6 mL , 3 mL
E-IR-R220	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0)	10 mL , 3 mL
E-IR-R221	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0)	10 mL , 3 mL
E-AB-1076	Goat Anti-Mouse IgG(H+L)(Elab Fluor® 647 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1196	HRP-Goat Anti-Mouse IgG(H+L) preadsorbed	500 μL , 120 μL , 1 mL