AMPK alpha1/2 Polyclonal Antibody
Price:
- 宿主： Rabbit
- 反应性： Human;Mouse;Rat;Monkey
- 应用性： WB;IHC-p
| Verified Samples |
Verified Samples in WB: K562 Verified Samples in IHC: Rat lung, Mouse testis |
| Dilution | WB 1:500-1:2000, IHC 1:100-1:300 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthesized peptide derived from human AMPKα1/2 around the non-phosphorylation site of Thr183/172. |
| Abbre | AMPKα1/2 |
| Synonyms | 5'-AMP-activated protein kinase catalytic subunit alpha-1, ACACA kinase, AMPK, AMPK subunit alpha-1, AMPK1, Acetyl-CoA carboxylase kinase, HMGCR kinase, Hydroxymethylglutaryl-CoA reductase kinase, PRKAA1, PRKAA2, Tau-protein kinase PRKAA1 |
| Swissprot | |
| Calculated MW | 62 kDa |
| Observed MW | 63 kDa |
| Cellular Localization | Cytosol, Nucleus, nuclear speck, nucleoplasm, nucleus, Plasma Membrane, apical plasma membrane, Other locations: cytoplasm, intracellular, nucleotide-activated protein kinase complex. |
| Concentration | 1 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH 7.4. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Cardiovascular, Metabolism, Neuroscience, Signal Transduction |
| Conjugation | Unconjugated |
| Background | AMPK (for 5'-AMP-activated protein kinase) is a heterotrimeric complex comprising a catalytic α subunit and regulatory β and γ subunits. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase, and inhibition of dephosphorylation.Activated AMPK can phosphorylate and regulate in vivo hydroxymethylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively. |
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Inhibition of lactate dehydrogenase A by diclofenac sodium induces apoptosis in HeLa cells through activation of AMPK
IF: 5.500 -
Cytarabine prevents neuronal damage by enhancing AMPK to stimulate PINK1 / Parkin-involved mitophagy in Parkinson's disease model
IF: 4.200 -
Elucidation of anti-human melanoma and anti-aging mechanisms of compounds from green seaweed Caulerpa racemosa
IF: 3.800
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| E-AB-1043 | Streptavidin(peroxidase/HRP conjugated) | 500 μL , 120 μL , 60 μL , 30 μL , 1 mL |
| E-AB-1194 | HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed | 500 μL , 120 μL , 1 mL |
| E-IR-R217 | 2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution) | 50 mL , 18 mL , 6 mL , 3 mL |
| E-IR-R220 | Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0) | 10mL , 3mL |
| E-IR-R221 | Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0) | 10mL , 3mL |
| E-IR-R304A | Western Blot Detection Kit | 50 Assays |
| E-IR-R304B | High Accuracy and Absorbability Western Blot Detection Kit | 50 Assays |
