ATPase Activity Assay Kit
中文名称:ATP酶比色法测试盒

Price:
Size:
96 T (40 samples)
- 样本类型: 动物组织;细胞
- 检测仪器及波长: 酶标仪(630-650 nm,最佳检测波长为640 nm)
- 研究领域: 氧化应激
该指标在测定组织或细胞样本时,需要测定样本的总蛋白含量,推荐使用BCA法测蛋白,货号E-BC-K318-M
说明书或网站上未涉及的样本类型请先跟技术支持沟通,可进行售前验证
根据需测样本数,试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量,请点击按钮 计算
检测原理 |
ATP酶将三磷酸腺苷(ATP) 催化水解为二磷酸腺苷(ADP) 和无机磷,然后通过定磷法确定单位时间内生成无机磷的含量来确定酶活大小。
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灵敏度 | 0.06 U/L |
检测范围 | 0.06-2.75 U/L |
精密度 | 批间差:0.05 | 批内差:0.04 |
样本量 | 200 μL(tissue/cell homogenate) |
检测时间 | 1 h 20 min |
检测仪器及波长 | 酶标仪(630-650 nm,最佳检测波长为640 nm) |
保存条件 | 该产品可在 2-8°C避光条件下保存6个月。 |
有效期 | 6个月 |
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E-BC-K122-S | H+K+-ATPase Activity Assay Kit | 200 Assays (100 samples) , 100 Assays (50 samples) |
E-BC-K212-M | Ca2+-ATPase Activity Assay Kit | 96 T (46 samples) , 48 T (22 samples) |
E-BC-K539-M | Na+k+-ATPase Activity Assay Kit | 96 T (40 samples) |
Q1:这个试剂盒的显色剂配制后出现特别粘稠的情况,是什么原因?
该试剂盒的试剂四(显色剂)在温度特别低的情况下会有这种粘稠物析出来,是正常情况,使用前先在37℃下孵育30min再使用。