beta actin Monoclonal Antibody
Price:
Size:
100 μL
25 μL
- 宿主： Mouse
- 反应性： Human;Mouse;Rat;Zebrafish
- 应用性： WB;IF
| Verified Samples |
Verified Samples in WB: PANC-1, 293F, Human placenta, SP2/0, Mouse kidney Verified Samples in IF: Zebrafish, 293 |
| Dilution | WB 1:1000-2000, IF 1:100-200 |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Immunogen | Recombinant human Beta actin protein |
| Abbre | beta actin |
| Synonyms | A26C1A, Actin beta, Actin cytoplasmic 1, Actx, b actin, ACTB |
| Swissprot | |
| Calculated MW | 43 kDa |
| Observed MW | 43 kDa |
| Cellular Localization | Cytoplasm, Cytoskeleton, Nucleus. |
| Concentration | 1 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Protein A/G Purification |
| Clone No. | 2B2 |
| Conjugation | Unconjugated |
| Background | Beta-actin (ACTB/ACTB) is one of six different actin isoforms in humans. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. Beta-actin gene is stably and constitutively expressed at high levels in most tissues and cells, and commonly used as a loading control for western blot and as a control for qPCR. |
Western Blotting of cell/tissue lysates with anti-Beta Actin monoclonal antibody at dilution of 1:2000.
Lane1: PANC-1 cell lysates, Lane2: 293F cell lysates, Lane3: Human placenta lysates, Lane4: Mouse SP2/0 celll ysates, Lane5: Mouse kidney lysates.
Western blotting of Zebrafish lysates with anti-Beta Actin monoclonal antibody at dilution of 1:2000.
Immunofluorescence analysis of 293 cells with anti-beta actin monoclonal antibody at 1:100 dilution.
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