beta actin Monoclonal Antibody
Price:
Size:
100 μL
25 μL
- 宿主： Mouse
- 反应性： Human;Mouse;Rat;Zebrafish
- 应用性： WB;IF
| Verified Samples |
Verified Samples in WB: PANC-1, 293F, Human placenta, SP2/0, Mouse kidney Verified Samples in IF: Zebrafish, 293 |
| Dilution | WB 1:1000-2000, IF 1:100-200 |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Immunogen | Recombinant human Beta actin protein |
| Abbre | beta actin |
| Synonyms | A26C1A, Actin beta, Actin cytoplasmic 1, Actx, b actin, ACTB |
| Swissprot | |
| Calculated MW | 43 kDa |
| Observed MW | 43 kDa |
| Cellular Localization | Cytoplasm, Cytoskeleton, Nucleus. |
| Concentration | 1 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Protein A/G Purification |
| Clone No. | 2B2 |
| Conjugation | Unconjugated |
| Background | Beta-actin (ACTB/ACTB) is one of six different actin isoforms in humans. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. Beta-actin gene is stably and constitutively expressed at high levels in most tissues and cells, and commonly used as a loading control for western blot and as a control for qPCR. |
Western Blotting of cell/tissue lysates with anti-Beta Actin monoclonal antibody at dilution of 1:2000.
Lane1: PANC-1 cell lysates, Lane2: 293F cell lysates, Lane3: Human placenta lysates, Lane4: Mouse SP2/0 celll ysates, Lane5: Mouse kidney lysates.
Western blotting of Zebrafish lysates with anti-Beta Actin monoclonal antibody at dilution of 1:2000.
Immunofluorescence analysis of 293 cells with anti-beta actin monoclonal antibody at 1:100 dilution.
-
Paeonol protects against acute pancreatitis by Nrf2 and NF-κB pathways in mice
IF: 4.810Journal： JOURNAL OF PHARMACY AND PHARMACOLOGY (2022)PMID：36170125Reactivity：MouseSample Type：pancreatic tissue -
Hypermethylation of the GRHL2 promoter region is associated with ovarian endometriosis
IF: 3.923 -
Chuanzhitongluo regulates microglia polarization and inflammatory response in acute ischemic stroke
IF: 3.715 -
Thymol Ameliorates Aspergillus fumigatus Keratitis by Downregulating the TLR4/ MyD88/ NF-kB/ IL-1β Signal Expression and Reducing Necroptosis and Pyroptosis
IF: 3.277Journal： JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY (2022)Reactivity：MouseSample Type：cornea -
Lipoxin A4 alleviates inflammation in Aspergillus fumigatus–stimulated human corneal epithelial cells by Nrf2/HO-1 signaling pathway
IF: 2.711 -
Royal jelly regulates the caspase, Bax and COX-2, TNF-α protein pathways in the fluoride exposed lung damage in rats
IF: 2.586 -
Lenvatinib Inhibits AKT/NF-κB Signaling and Induces Apoptosis Through Extrinsic/Intrinsic Pathways in Non-small Cell Lung Cancer
IF: 2.480 -
Perillaldehyde Ameliorates Aspergillus fumigatus Keratitis by Activating the Nrf2/HO-1 Signaling Pathway and Inhibiting Dectin-1-Mediated Inflammation
IF: 3.470Journal： INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE (2020)Reactivity：Human -
Role of corticotropin-releasing hormone in the impact of chronic stress during pregnancy on inducing depression in male offspring mice
IF: 2.733Journal： BRAIN RESEARCH (2020)Reactivity：MouseSample Type：hippocampal tissue -
Morpho-functional effects of different universal dental adhesives on human gingival fibroblasts: an in vitro study
IF: 1.840
积分商城
微信公众号
