beta actin Monoclonal Antibody-Elabscience伊莱瑞特【官网】

beta actin Monoclonal Antibody

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货号：E-AB-48018
文献（42）
说明书

说明书

Price: ¥780.00 ¥300.00

Size:

100 μL 25 μL

宿主： Mouse
反应性： Human;Mouse;Rat;Zebrafish
应用性： WB;IF

Verified Samples	Verified Samples in WB: PANC-1, 293F, Human placenta, SP2/0, Mouse kidney Verified Samples in IF: Zebrafish, 293
Dilution	WB 1:1000-2000, IF 1:100-200
Clonality	Monoclonal
Isotype	IgG2a
Immunogen	Recombinant human Beta actin protein
Abbre	beta actin
Synonyms	A26C1A, Actin beta, Actin cytoplasmic 1, Actx, b actin, ACTB
Swissprot	Q7ZVF9
Calculated MW	43 kDa
Observed MW	43 kDa
Cellular Localization	Cytoplasm, Cytoskeleton, Nucleus.
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4
Purification Method	Protein A/G Purification
Clone No.	2B2
Conjugation	Unconjugated
Background	Beta-actin (ACTB/ACTB) is one of six different actin isoforms in humans. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. Beta-actin gene is stably and constitutively expressed at high levels in most tissues and cells, and commonly used as a loading control for western blot and as a control for qPCR.

Western Blotting of cell/tissue lysates with anti-Beta Actin monoclonal antibody at dilution of 1:2000. Lane1: PANC-1 cell lysates, Lane2: 293F cell lysates, Lane3: Human placenta lysates, Lane4: Mouse SP2/0 celll ysates, Lane5: Mouse kidney lysates.

Western blotting of Zebrafish lysates with anti-Beta Actin monoclonal antibody at dilution of 1:2000.

Immunofluorescence analysis of 293 cells with anti-beta actin monoclonal antibody at 1:100 dilution.

Endogenous ET-1 promotes ANP secretion through activation of COX2-L–PGDS–PPARγ signaling in hypoxic beating rat atria

IF: 2.659

Journal： PEPTIDES (2019)

DOI：10.1016/j.peptides.2019.170150

PMID：31541683

Reactivity：Rat

Sample Type：left atrial
First evidence of DAAM1 localization in mouse seminal vesicles and its possible involvement during regulated exocytosis

IF: 1.313

Journal： COMPTES RENDUS BIOLOGIES (2018)

DOI：10.1016/j.crvi.2018.03.001

Reactivity：Mouae,Rat

Sample Type：seminal vesicles,testes

E-AB-1001	Goat Anti-Mouse IgG(H+L)(peroxidase/HRP conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1011	Goat Anti-Mouse IgG(H+L)(Cyanine3 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1015	Goat Anti-Mouse IgG(H+L)(FITC conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1024	Goat Anti-Mouse IgG(H+L)(Biotin conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1043	Streptavidin(peroxidase/HRP conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1059	Goat Anti-Mouse IgG(H+L)(Elab Fluor® 594 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1076	Goat Anti-Mouse IgG(H+L)(Elab Fluor® 647 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1196	HRP-Goat Anti-Mouse IgG(H+L) preadsorbed	500 μL , 120 μL , 1 mL
E-IR-R217	2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution)	50 mL , 18 mL , 6 mL , 3 mL
E-IR-R220	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0)	10mL , 3mL
E-IR-R221	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0)	10mL , 3mL
E-IR-R304A	Western Blot Detection Kit	50 Assays
E-IR-R304B	High Accuracy and Absorbability Western Blot Detection Kit	50 Assays