beta actin Polyclonal Antibody
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Price:
Size:
100 μL
25 μL
- 宿主： Rabbit
- 反应性： Human;Mouse;Rat
- 应用性： WB;IHC
| Verified Samples |
Verified Samples in WB: A431, RAW264.7, Rat brain, Mouse brain Verified Samples in IHC: Human colon, Human breast cancer, Mouse heart, Rat heart |
| Dilution | WB 1:3000-1:30000, IHC 1:100-1:300 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant Zebrafish Actin, cytoplasmic 2 protein |
| Abbre | ACTBB |
| Synonyms | A26C1A, Actin beta, Actin cytoplasmic 1, Actx, b actin, ACTB |
| Swissprot | |
| Calculated MW | 41 kDa |
| Observed MW | 41 kDa |
| Cellular Localization | Cytoplasm, Cytoskeleton, Nucleus. |
| Concentration | 1 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Antigen Affinity Purification |
| Research Areas | Isotype, Tags and Cell markers, Signal Transduction |
| Conjugation | Unconjugated |
| Background | Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins. |
Western Blot analysis of A431 cells using beta actin Polyclonal Antibody at dilution of 1:2000
Western Blot analysis of Rat brain and Mouse brain using beta actin Polyclonal Antibody at dilution of 1:2000
Western Blot analysis of Raw264.7 cells using beta actin Polyclonal Antibody at dilution of 1:2000
Immunohistochemistry of paraffin-embedded Human colon using beta actin Polyclonal Antibody at dilution of 1:200
Immunohistochemistry of paraffin-embedded Human breast cancer using beta actin Polyclonal Antibody at dilution of 1:200
Immunohistochemistry of paraffin-embedded Mouse heart using beta actin Polyclonal Antibody at dilution of 1:200
Immunohistochemistry of paraffin-embedded Rat heart using beta actin Polyclonal Antibody at dilution of 1:200
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