beta actin Polyclonal Antibody（E-AB-40338）|-Elabscience伊莱瑞特【官网】

beta actin Polyclonal Antibody

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货号：E-AB-40338
文献（119）
说明书

说明书

Price: ¥780.00 ¥300.00

Size:

100 μL 25 μL

宿主： Rabbit
反应性： Human;Mouse;Rat
应用性： WB;IHC

Verified Samples	Verified Samples in WB: A431, RAW264.7, Rat brain, Mouse brain Verified Samples in IHC: Human colon, Human breast cancer, Mouse heart, Rat heart
Dilution	WB 1:3000-1:30000, IHC 1:100-1:300
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant Zebrafish Actin, cytoplasmic 2 protein
Abbre	ACTBB
Synonyms	A26C1A, Actin beta, Actin cytoplasmic 1, Actx, b actin, ACTB
Swissprot	Q7ZVF9
Calculated MW	41 kDa
Observed MW	41 kDa
Cellular Localization	Cytoplasm, Cytoskeleton, Nucleus.
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.05% Proclin300, 1% protective protein and 50% glycerol, pH7.4
Purification Method	Antigen Affinity Purification
Research Areas	Isotype, Tags and Cell markers, Signal Transduction
Conjugation	Unconjugated
Background	Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins.

Western Blot analysis of A431 cells using beta actin Polyclonal Antibody at dilution of 1:2000

Western Blot analysis of Rat brain and Mouse brain using beta actin Polyclonal Antibody at dilution of 1:2000

Western Blot analysis of Raw264.7 cells using beta actin Polyclonal Antibody at dilution of 1:2000

Immunohistochemistry of paraffin-embedded Human colon using beta actin Polyclonal Antibody at dilution of 1:200

Immunohistochemistry of paraffin-embedded Human breast cancer using beta actin Polyclonal Antibody at dilution of 1:200

Immunohistochemistry of paraffin-embedded Mouse heart using beta actin Polyclonal Antibody at dilution of 1:200

Immunohistochemistry of paraffin-embedded Rat heart using beta actin Polyclonal Antibody at dilution of 1:200

Galectin-3 plays an important pro-inflammatory role in A. fumigatus keratitis by recruiting neutrophils and activating p38 in neutrophils

IF: 4.932

Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2021)

DOI：10.1016/j.intimp.2021.107706

PMID：33933850

Reactivity：Mouse

Sample Type：corneas and
IL-36α Exerts Proinflammatory Effects in Aspergillus fumigatus Keratitis of Mice Through the Pathway of IL-36α/IL-36R/NF-κB

IF: 4.799

Journal： INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE (2021)

DOI：10.1167/iovs.62.4.16

PMID：33851975

Reactivity：Mouse

Sample Type：Cell sample
Baicalein Inhibits Gastric Cancer Cell Proliferation and Migration through a FAK Interaction via AKT/mTOR Signaling

IF: 4.667

Journal： AMERICAN JOURNAL OF CHINESE MEDICINE (2021)

DOI：10.1142/S0192415X21500245

PMID：33641654

Reactivity：Human
4-Methylumbelliferone treatment and hyaluronan inhibition as a therapeutic strategy for chronic prostatitis

IF: 4.104

Journal： PROSTATE (2021)

DOI：10.1002/pros.24205

PMID：34320251

Reactivity：Mouse

Sample Type：prostate
Lenvatinib Inhibits AKT/NF-κB Signaling and Induces Apoptosis Through Extrinsic/Intrinsic Pathways in Non-small Cell Lung Cancer

IF: 2.480

Journal： ANTICANCER RESEARCH (2021)

DOI：10.21873/anticanres.14757

Reactivity：Human
α- MSH Plays Anti-inflammatory and Anti-fungal Role in Aspergillus Fumigatus Keratitis

IF: 2.424

Journal： CURRENT EYE RESEARCH (2021)

DOI：10.1080/02713683.2021.2006235

PMID：34766863

Reactivity：Mouse

Sample Type：corneas
lncRNA STEAP3-AS1 Modulates Cell Cycle Progression via Affecting CDKN1C Expression through STEAP3 in Colon Cancer

IF: 7.032

Journal： Molecular Therapy-Nucleic Acids (2020)

DOI：10.1016/j.omtn.2020.06.011

PMID：32679543

Reactivity：Human

Sample Type：Cell lysate
miR-3666 inhibits development of hepatic steatosis by negatively regulating PPARγ

IF: 4.519

Journal： BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS (2020)

DOI：10.1016/j.bbalip.2020.158777

PMID：32755726

Reactivity：Human

Sample Type：Cell sample
MicroRNA-4316 inhibits gastric cancer proliferation and migration via directly targeting VEGF-A

IF: 4.175

Journal： Cancer Cell International (2020)

DOI：10.1186/s12935-020-1132-3

PMID：32123520

Reactivity：Human
Anti-inflammatory and analgesic activities of indigo through regulating the IKKβ/IκB/NF-κB pathway in mice

IF: 4.171

Journal： Food & Function (2020)

DOI：10.1039/C9FO02574J

PMID：33084638

Reactivity：Mouse

Sample Type：skin

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