beta actin Polyclonal Antibody（E-AB-40338）|-Elabscience伊莱瑞特【官网】

beta actin Polyclonal Antibody

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货号：E-AB-40338
文献（119）
说明书

说明书

Price: ¥780.00 ¥300.00

Size:

100 μL 25 μL

宿主： Rabbit
反应性： Human;Mouse;Rat
应用性： WB;IHC

Verified Samples	Verified Samples in WB: A431, RAW264.7, Rat brain, Mouse brain Verified Samples in IHC: Human colon, Human breast cancer, Mouse heart, Rat heart
Dilution	WB 1:3000-1:30000, IHC 1:100-1:300
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant Zebrafish Actin, cytoplasmic 2 protein
Abbre	ACTBB
Synonyms	A26C1A, Actin beta, Actin cytoplasmic 1, Actx, b actin, ACTB
Swissprot	Q7ZVF9
Calculated MW	41 kDa
Observed MW	41 kDa
Cellular Localization	Cytoplasm, Cytoskeleton, Nucleus.
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.05% Proclin300, 1% protective protein and 50% glycerol, pH7.4
Purification Method	Antigen Affinity Purification
Research Areas	Isotype, Tags and Cell markers, Signal Transduction
Conjugation	Unconjugated
Background	Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins.

Western Blot analysis of A431 cells using beta actin Polyclonal Antibody at dilution of 1:2000

Western Blot analysis of Rat brain and Mouse brain using beta actin Polyclonal Antibody at dilution of 1:2000

Western Blot analysis of Raw264.7 cells using beta actin Polyclonal Antibody at dilution of 1:2000

Immunohistochemistry of paraffin-embedded Human colon using beta actin Polyclonal Antibody at dilution of 1:200

Immunohistochemistry of paraffin-embedded Human breast cancer using beta actin Polyclonal Antibody at dilution of 1:200

Immunohistochemistry of paraffin-embedded Mouse heart using beta actin Polyclonal Antibody at dilution of 1:200

Immunohistochemistry of paraffin-embedded Rat heart using beta actin Polyclonal Antibody at dilution of 1:200

Kupffer cell depletion attenuates IL-6/STAT3 mediates hepatocyte apoptosis in immunological liver injury of trichloroethylene sensitized mice

IF: 3.943

Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2020)

DOI：10.1016/j.intimp.2020.106897

PMID：32822909

Reactivity：Mouse

Sample Type：liver tissue
Hepatic cholesterol synthesis and lipoprotein levels impaired by dietary fructose and saturated fatty acids in mice: Insight on PCSK9 and CD36

IF: 3.639

Journal： NUTRITION (2020)

DOI：10.1016/j.nut.2020.110954

PMID：32862122

Reactivity：Mouse

Sample Type：Tissue homogenate
Perillaldehyde Ameliorates Aspergillus fumigatus Keratitis by Activating the Nrf2/HO-1 Signaling Pathway and Inhibiting Dectin-1-Mediated Inflammation

IF: 3.470

Journal： INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE (2020)

DOI：10.1167/iovs.61.6.51

Reactivity：Human
NLRC5 negatively regulates inflammatory responses in LPS-induced acute lung injury through NF-κB and p38 MAPK signal pathways

IF: 3.347

Journal： TOXICOLOGY AND APPLIED PHARMACOLOGY (2020)

DOI：10.1016/j.taap.2020.115150

PMID：32710960

Reactivity：Mouse

Sample Type：Cell sample
Role of corticotropin-releasing hormone in the impact of chronic stress during pregnancy on inducing depression in male offspring mice

IF: 2.733

Journal： BRAIN RESEARCH (2020)

DOI：10.1016/j.brainres.2020.147029

Reactivity：Mouse

Sample Type：hippocampal tissue
Anticancer Efficacy and Mechanism of Amentoflavone for Sensitizing Oral Squamous Cell Carcinoma to Cisplatin

IF: 1.994

Journal： ANTICANCER RESEARCH (2020)

DOI：10.21873/anticanres.14695

PMID：33288565

Reactivity：Human

Sample Type：Cell sample
Green tea polyphenols decrease weight gain, ameliorate alteration of gut microbiota, and mitigate intestinal inflammation in canines with high-fat-diet-induced obesity

IF: 4.490

Journal： JOURNAL OF NUTRITIONAL BIOCHEMISTRY (2019)

DOI：10.1016/j.jnutbio.2019.108324

PMID：32004926

Reactivity：Canine

Sample Type：Tissue homogenate
DC-SIGN mediates gastric cancer progression by regulating the JAK2/STAT3 signaling pathway and affecting LncRNA RP11-181G12.2 expression

IF: 3.743

Journal： BIOMEDICINE & PHARMACOTHERAPY (2019)

DOI：10.1016/j.biopha.2019.109644

PMID：31766099

Reactivity：Human

Sample Type：Cell lysate
MAST3 modulates the inflammatory response and proliferation of fibroblast-like synoviocytes in rheumatoid arthritis

IF: 3.361

Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2019)

DOI：10.1016/j.intimp.2019.105900

PMID：31644963

Reactivity：Rat

Sample Type：Synovial
Vitexin alleviates non-alcoholic fatty liver disease by activating AMPK in high fat diet fed mice

IF: 2.705

Journal： BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2019)

DOI：10.1016/j.bbrc.2019.08.139

PMID：31472955

Reactivity：Mouse

Sample Type：Tissue homogenate

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