beta actin Polyclonal Antibody（E-AB-40338）|-Elabscience伊莱瑞特【官网】

beta actin Polyclonal Antibody

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货号：E-AB-40338
文献（119）
说明书

说明书

Price: ¥780.00 ¥300.00

Size:

100 μL 25 μL

宿主： Rabbit
反应性： Human;Mouse;Rat
应用性： WB;IHC

Verified Samples	Verified Samples in WB: A431, RAW264.7, Rat brain, Mouse brain Verified Samples in IHC: Human colon, Human breast cancer, Mouse heart, Rat heart
Dilution	WB 1:3000-1:30000, IHC 1:100-1:300
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant Zebrafish Actin, cytoplasmic 2 protein
Abbre	ACTBB
Synonyms	A26C1A, Actin beta, Actin cytoplasmic 1, Actx, b actin, ACTB
Swissprot	Q7ZVF9
Calculated MW	41 kDa
Observed MW	41 kDa
Cellular Localization	Cytoplasm, Cytoskeleton, Nucleus.
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.05% Proclin300, 1% protective protein and 50% glycerol, pH7.4
Purification Method	Antigen Affinity Purification
Research Areas	Isotype, Tags and Cell markers, Signal Transduction
Conjugation	Unconjugated
Background	Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins.

Western Blot analysis of A431 cells using beta actin Polyclonal Antibody at dilution of 1:2000

Western Blot analysis of Rat brain and Mouse brain using beta actin Polyclonal Antibody at dilution of 1:2000

Western Blot analysis of Raw264.7 cells using beta actin Polyclonal Antibody at dilution of 1:2000

Immunohistochemistry of paraffin-embedded Human colon using beta actin Polyclonal Antibody at dilution of 1:200

Immunohistochemistry of paraffin-embedded Human breast cancer using beta actin Polyclonal Antibody at dilution of 1:200

Immunohistochemistry of paraffin-embedded Mouse heart using beta actin Polyclonal Antibody at dilution of 1:200

Immunohistochemistry of paraffin-embedded Rat heart using beta actin Polyclonal Antibody at dilution of 1:200

Melatonin protects bone against cadmium-induced toxicity via activation of Wnt/β-catenin signaling pathway

IF: 2.276

Journal： TOXICOLOGY MECHANISMS AND METHODS (2019)

DOI：10.1080/15376516.2019.1701595

PMID：31809235

Reactivity：Rat

Sample Type：Femoral bone tissue
Inhibition of the hypoxia-induced factor-1α and vascular endothelial growth factor expression through ginsenoside Rg3 in human gastric cancer cells

IF: 1.392

Journal： Journal of Cancer Research and Therapeutics (2019)

DOI：10.4103/jcrt.JCRT_77_17

PMID：31939450

Reactivity：Human
Synergetic Impact of Combined 5-Fluorouracil and Rutin on Apoptosis in PC3 Cancer Cells through the Modulation of P53 Gene Expression

Journal： Advanced Pharmaceutical Bulletin (2019)

DOI：10.15171/apb.2019.055

PMID：31592435

Reactivity：Human

Sample Type：Cell lysate
TRAF6 Affects RAC1 Expression and Apoptosis in SK-Hep1 Cells

IF: 1.802

Journal： Chinese Medicine (2018)

DOI：10.4236/cm.2018.94011

Reactivity：Human
Paeoniflorin Inhibits Migration- and Invasion-Promoting Capacities of Gastric Cancer Associated Fibroblasts

IF: 1.346

Journal： Chinese Journal of Integrative Medicine (2018)

DOI：10.1007/s11655-018-2985-3

PMID：30361813

Reactivity：Human

Sample Type：gastric carcinoma AGS cells
First evidence of DAAM1 localization in mouse seminal vesicles and its possible involvement during regulated exocytosis

IF: 1.313

Journal： COMPTES RENDUS BIOLOGIES (2018)

DOI：10.1016/j.crvi.2018.03.001

Reactivity：Mouae,Rat

Sample Type：seminal vesicles,testes
Astragaloside IV inhibits pathological functions of gastric cancer-associated fibroblasts

IF: 3.365

Journal： WORLD JOURNAL OF GASTROENTEROLOGY (2017)

DOI：10.3748/wjg.v23.i48.8512

PMID：29358859

Reactivity：Human

Sample Type：Cell lysate
Effects of acrylonitrile-induced oxidative stress on testicular apoptosis through activation of NF-κB signaling pathway in male sprague dawley rats

IF: 2.829

Journal： American Journal of Translational Research (2017)

DOI：PMID:28979696

PMID：28979696

Reactivity：Rat

Sample Type：testicular tissue
Protective Effects of Diallyl Sulfide Against Ethanol-Induced Injury in Rat Adipose Tissue and Primary Human Adipocytes

IF: 2.716

Journal： ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH (2017)

DOI：10.1111/acer.13398

PMID：28414868

Reactivity：Rat

Sample Type：Adipose tissue

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