beta actin Polyclonal Antibody（E-AB-40338）|-Elabscience伊莱瑞特【官网】

beta actin Polyclonal Antibody

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货号：E-AB-40338
文献（119）
说明书

说明书

Price: ¥780.00 ¥300.00

Size:

100 μL 25 μL

宿主： Rabbit
反应性： Human;Mouse;Rat
应用性： WB;IHC

Verified Samples	Verified Samples in WB: A431, RAW264.7, Rat brain, Mouse brain Verified Samples in IHC: Human colon, Human breast cancer, Mouse heart, Rat heart
Dilution	WB 1:3000-1:30000, IHC 1:100-1:300
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant Zebrafish Actin, cytoplasmic 2 protein
Abbre	ACTBB
Synonyms	A26C1A, Actin beta, Actin cytoplasmic 1, Actx, b actin, ACTB
Swissprot	Q7ZVF9
Calculated MW	41 kDa
Observed MW	41 kDa
Cellular Localization	Cytoplasm, Cytoskeleton, Nucleus.
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.05% Proclin300, 1% protective protein and 50% glycerol, pH7.4
Purification Method	Antigen Affinity Purification
Research Areas	Isotype, Tags and Cell markers, Signal Transduction
Conjugation	Unconjugated
Background	Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins.

Western Blot analysis of A431 cells using beta actin Polyclonal Antibody at dilution of 1:2000

Western Blot analysis of Rat brain and Mouse brain using beta actin Polyclonal Antibody at dilution of 1:2000

Western Blot analysis of Raw264.7 cells using beta actin Polyclonal Antibody at dilution of 1:2000

Immunohistochemistry of paraffin-embedded Human colon using beta actin Polyclonal Antibody at dilution of 1:200

Immunohistochemistry of paraffin-embedded Human breast cancer using beta actin Polyclonal Antibody at dilution of 1:200

Immunohistochemistry of paraffin-embedded Mouse heart using beta actin Polyclonal Antibody at dilution of 1:200

Immunohistochemistry of paraffin-embedded Rat heart using beta actin Polyclonal Antibody at dilution of 1:200

Oroxylin A alleviates pyroptosis and apoptosis in human corneal epithelial cells under hyperosmotic stress by activating the SIRT3-SOD2/HIF-1α pathway

IF: 3.0

Journal： EXPERIMENTAL EYE RESEARCH (2025)

DOI：10.1016/j.exer.2025.110345

Reactivity：Human
Rutin resists Aspergillus fumigatus keratitis by activating Nrf2/HO-1 pathway, inhibiting Dectin-1/p-Syk pathway and affecting fungal structures

IF: 3.0

Journal： EXPERIMENTAL EYE RESEARCH (2025)

DOI：10.1016/j.exer.2025.110323

Reactivity：Mouse

Sample Type：cornea tissue
Protective effect of conditioned medium derived from melatonin-stimulated stem cells from the apical papilla on glutamate-induced neurotoxicity in PC12 cells

IF: 2.9

Journal： NEUROSCIENCE (2025)

DOI：10.1016/j.neuroscience.2025.02.031

Reactivity：Rat
Effects of Ellagic Acid and Berberine on Hind Limb Ischemia Reperfusion Injury: Pathways of Apoptosis, Pyroptosis, and Oxidative Stress

IF: 2.4

Journal： Medicina-Lithuania (2025)

DOI：10.3390/medicina61030451

Reactivity：Rat

Sample Type：skeletal muscle tissue
Effects of Ischemic Post-conditioning and Thymoquinone on Liver Ischemia Reperfusion Injury in Rats

IF: 1.5

Journal： Bratislava Medical Journal-Bratislavske Lekarske Listy (2025)

DOI：10.1007/s44411-025-00150-4

Reactivity：Rat

Sample Type：liver tissue
A Novel Homozygous Germline Mutation in Transferrin Receptor 1 (TfR1) Leads to Combined Immunodeficiency and Provides New Insights into Iron-Immunity Axis

IF: 9.1

Journal： JOURNAL OF CLINICAL IMMUNOLOGY (2024)

DOI：10.1007/s10875-024-01658-0

Sample Type：Peripheral blood mononuclear cell
Orally fast dissolving α-lipoic acid electrospun nanofibers mitigates lipopolysaccharide induced inflammation in RAW 264.7 macrophages

IF: 8.2

Journal： INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2024)

DOI：10.1016/j.ijbiomac.2024.130623

Sample Type：RAW 264.7 cell
Galloyl-oligochitosan nano-vehicles for effective and controlled propolis delivery targeting upgrading its antioxidant and antiproliferative potential

IF: 7.7

Journal： INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2024)

DOI：10.1016/j.ijbiomac.2024.132283

Reactivity：Human
Graphene oxide nanoribbons conjugated with 1, 2-distearoyl-sn-glycero-3 phosphoethanolamine-poly (ethylene glycol)-transferrin enhanced targeted delivery and cytotoxicity of raloxifene against breast cancer

IF: 7.7

Journal： INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2024)

DOI：10.1016/j.ijbiomac.2024.134772

Reactivity：Human
Multifunctional natamycin modified chondroitin sulfate eye drops with anti-inflammatory, antifungal and tissue repair functions possess therapeutic effects on fungal keratitis in mice.

IF: 7.7

Journal： INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2024)

DOI：10.1016/j.ijbiomac.2024.135290

Reactivity：Mouse

Sample Type：corneas tissue

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